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J. Exp. Med.,
Volume 189, Number 4, February 15, 1999 735-740
By
From the Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
Lineage commitment in B lymphopoiesis remains poorly understood due to the inability to
clearly define newly committed B lineage progenitors and their multipotential descendants. We
examined the potential of three recently described progenitor populations in adult mouse bone
marrow to differentiate into each hematopoietic lineage. The earliest of these, termed fraction
(Fr.) A0, exhibited myeloid, erythroid, and B and T lymphoid progenitor activity and included
individual cells with myeloid/B lymphoid potential. In sharp contrast, two later populations,
termed Frs. A1 and A2 and characterized by surface B220 expression and transcription of the
germline immunoglobulin heavy chain (IgH) locus, lacked progenitor activity for all hematopoietic lineages except B lymphocytes. These observations, together with single cell polymerase chain reaction analysis showing a lack of DHJH rearrangements in each population and
experiments showing identical precursor potentials when these populations were derived from
recombination activating gene (Rag)-1
/ and JH/ mice, demonstrate that commitment to
the B lymphoid lineage occurs before and independently of VHDHJH recombination.
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