© The Rockefeller University Press, 0022-1007/1999/2/693/ $5.00
The Journal of Experimental Medicine, Volume 189, Number 4, February 15, 1999 693-700
CD34+ Hematopoietic Stem Cells Exert Accessory Function in Lipopolysaccharide-induced T Cell Stimulation and CD80 Expression on Monocytes
Taila Mattern*,
Gundolf Girroleit*,
Hans-Dieter Flad*,
Ernst T. Rietschel
, and
Artur J. Ulmer*
From the * Department of Immunology and Cell Biology, and the
Department of Immunochemistry and Biochemical Microbiology, Research Center Borstel, 23845 Borstel, Germany
CD34+ hematopoietic stem cells, which circulate in peripheral blood with very low frequency, exert essential accessory function during lipopolysaccharide (LPS)-induced human T lymphocyte activation, resulting in interferon
production and proliferation. In contrast, stimulation of T cells by "conventional" recall antigens is not controlled by blood stem cells. These conclusions are based on the observation that depletion of CD34+ blood stem cells results in a loss of LPS-induced T cell stimulation as well as reduced expression of CD80 antigen on monocytes. The addition of CD34-enriched blood stem cells resulted in a recovery of reactivity of T cells and monocytes to LPS. Blood stem cells could be replaced by the hematopoietic stem cell line KG-1a. These findings may be of relevance for high risk patients treated with stem cells or stem cell recruiting compounds and for patients suffering from endotoxin-mediated diseases.
Key Words: antigen presentation immunity, cellular immunoreactivity immunocompetence lymphocyte cooperation
Address correspondence to Taila Mattern, Research Center Borstel, Center for Medicine and Biosciences, Parkallee 22, D-23845 Borstel, Germany. Phone: 49-4537-188-448; Fax: 49-4537-188-435; E-mail: ajulmer{at}fz-borstel.de
This work was financially supported by the Deutsche Forschungsgemeinschaft (SFB 367, project C5) and the Fond der Chemischen Industrie (to H.D. Flad and E.T. Rietschel).
Abbreviations used: GaM, goat anti–mouse; HS, human serum; PPD, purified protein derivative; TT, tetanus toxoid.

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