The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1999/6/1973/ $5.00
The Journal of Experimental Medicine, Volume 189, Number 12, June 21, 1999 1973-1980

Articles

Susceptibility of Mice Deficient in CD1D or TAP1 to Infection with Mycobacterium tuberculosis

Samuel M. Behar*, Chris C. Dascher*, Michael J. Grusby*,{ddagger}, Chyung-Ru Wang§, and Michael B. Brenner*

From the * Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115; the {ddagger} Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115; and the § Gwen Knapp Center for Lupus and Immunology Research, University of Chicago, Chicago, Illinois 60637

Cellular immunity against Mycobacterium tuberculosis controls infection in the majority of infected humans. Studies in mice have delineated an important role for CD4+ T cells and cytokines including interferon {gamma} and tumor necrosis factor {alpha} in the response to infection with mycobacteria. Recently, the identification of CD8+ CD1-restricted T cells that kill M. tuberculosis organisms via granulysin and the rapid death after infection of β2 microglobulin deficient mice in humans has drawn attention to a critical role for CD8+ T cells. The nature of mycobacterial-specific CD8+ T cells has been an enigma because few have been identified in any species. Here, we delineate the contribution of class I MHC–restricted T cells in the defense against tuberculosis as transporter associated with antigen processing (TAP)1-deficient mice died rapidly, bore a greater bacterial burden, and had more severe tissue pathology than control mice. In contrast, CD1D–/– mice were not significantly different in their susceptibility to infection than control mice. This data demonstrates a critical role for TAP-dependent peptide antigen presentation and provides further evidence that class I MHC–restricted CD8+ T cells, the major T cell subset activated by this antigen processing pathway, play an essential role in immunity to tuberculosis.

Key Words: CD1 • CD8 • transporter associated with antigen processing • infection • Mycobacterium tuberculosis

Address correspondence to Samuel M. Behar, Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Smith Bldg., Rm. 516C, 1 Jimmy Fund Way, Boston, MA 02115. Phone: 617-525-1033; Fax: 617-525-1010; E-mail: sbehar{at}rics.bwh.harvard.edu

Abbreviations used: AFB, acid fast bacilli; β2m, β2 microglobulin; ER, endoplasmic reticulum; MST, mean survival time; TAP, transporter associated with antigen processing.


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