The Journal of Experimental Medicine
VeriKine-HS Human IFN-Beta
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
A correction to this article has been published: J. Exp. Med. 189 (8) 1361-1362
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 261K)
Right arrow PPT slides of all figures
Right arrow Correction (v189,p1361)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Novobrantseva, T. I.
Right arrow Articles by Ehlich, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Novobrantseva, T. I.
Right arrow Articles by Ehlich, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/1999/1/75/ $5.00
The Journal of Experimental Medicine, Volume 189, Number 1, January 4, 1999 75-88

Articles

Rearrangement and Expression of Immunoglobulin Light Chain Genes Can Precede Heavy Chain Expression during Normal B Cell Development in Mice

Tatiana I. Novobrantseva, Verena M. Martin, Roberta Pelanda, Werner Müller, Klaus Rajewsky, and Andreas Ehlich

From the Institute for Genetics, University of Cologne,Weyertal 121, 50931 Cologne, Germany

In mouse mutants incapable of expressing µ chains, V{kappa}J{kappa} joints are detected in the CD43+ B cell progenitors. In agreement with these earlier results, we show by a molecular single cell analysis that 4–7% of CD43+ B cell progenitors in wild-type mice rearrange immunoglobulin (Ig){kappa} genes before the assembly of a productive VHDHJH joint. Thus, µ chain expression is not a prerequisite to Ig{kappa} light chain gene rearrangements in normal development. Overall, ~15% of the total CD43+ B cell progenitor population carry Ig{kappa} gene rearrangements in wild-type mice. Together with the results obtained in the mouse mutants, these data fit a model in which CD43+ progenitors rearrange IgH and Ig{kappa} loci independently, with a seven times higher frequency in the former. In addition, we show that in B cell progenitors V{kappa}J{kappa} joining rapidly initiates {kappa} chain expression, irrespective of the presence of a µ chain.

Key Words: B cell development • bone marrow • immunoglobulin gene rearrangement

Address correspondence to Tatiana I. Novobrantseva, Institute for Genetics, University of Cologne, Weyertal 121, 50931 Cologne, Germany. Phone: 49-221-470-52-91; Fax: 49-221-470-51-85; E-mail: ntanya{at}mac.genetik.uni-koeln.de


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS