The Journal of Experimental Medicine
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A correction to this article has been published: J. Exp. Med. 189 (8) 1361-1362
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J. Exp. Med., Volume 189, Number 1, January 4, 1999 75-88

Rearrangement and Expression of Immunoglobulin Light Chain Genes Can Precede Heavy Chain Expression during Normal B Cell Development in Mice

By Tatiana I. Novobrantseva, Verena M. Martin, Roberta Pelanda, Werner Müller, Klaus Rajewsky, and Andreas Ehlich

From the Institute for Genetics, University of Cologne,Weyertal 121, 50931 Cologne, Germany

In mouse mutants incapable of expressing µ chains, Vkappa Jkappa joints are detected in the CD43+ B cell progenitors. In agreement with these earlier results, we show by a molecular single cell analysis that 4-7% of CD43+ B cell progenitors in wild-type mice rearrange immunoglobulin (Ig)kappa genes before the assembly of a productive VHDHJH joint. Thus, µ chain expression is not a prerequisite to Igkappa light chain gene rearrangements in normal development. Overall, ~15% of the total CD43+ B cell progenitor population carry Igkappa gene rearrangements in wild-type mice. Together with the results obtained in the mouse mutants, these data fit a model in which CD43+ progenitors rearrange IgH and Igkappa loci independently, with a seven times higher frequency in the former. In addition, we show that in B cell progenitors Vkappa Jkappa joining rapidly initiates kappa  chain expression, irrespective of the presence of a µ chain.

Key words: B cell developmentbone marrowimmunoglobulin gene rearrangement


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