|
|
|
|
|
|
|
||
J. Exp. Med.,
Volume 188, Number 9, November 2, 1998 1751-1756
By
From the * Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Interleukin (IL)-8, a C-X-C chemokine, activates integrin-mediated adhesion of neutrophils.
Presentation of IL-8 on the endothelial cell surface may promote leukocyte extravasation. We
found that cultured human microvascular endothelial cells from the intestine (HIMEC) and
from nasal polyps (PMEC), but not human umbilical vein endothelial cells (HUVEC), contained IL-8 in intracellular granules that coexpressed von Willebrand factor (vWf ). This observation was corroborated by the immunohistochemical observation of double-positive granules
(IL-8+vWf+) in vessels of small and large intestine, nasal mucosa, and skin, whereas umbilical
cords revealed no endothelial IL-8. After treatment of HIMEC or PMEC with histamine or
thrombin, a dramatic increase in supernatant IL-8 concentration was observed within 3 min,
whereas no increase in IL-8 was detected in supernatants of identically treated HUVEC cultures. Histamine or thrombin treatment also caused IL-8-containing granules to rapidly disappear from HIMEC. In HUVEC, IL-8-containing granules were inducible by treatment with
recombinant human IL-1
Surgical Department B, University of Oslo, The National Hospital,
Rikshospitalet, N-0027 Oslo, Norway
for 24 h; additional histamine treatment doubled IL-8 secretion
from HUVEC in the same rapid manner observed for mucosal EC. These data suggested that
IL-8 prestored in microvascular endothelial cells may provide a rapid pathway for specific activation of neutrophil adhesion at sites of acute inflammation.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
| TABLE OF CONTENTS |
|
