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Articles

Huaxian Chen^*, Michael Centola^*, Stephen F. Altschul, and Henry Metzger^*

From the ^* Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases and the National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20892

To characterize gene expression in activated mast cells more comprehensively than heretofore, we surveyed the changes in genetic transcripts by the method of serial analysis of gene expression in the RBL-2H3 line of rat mast cells before and after they were stimulated through their receptors with high affinity for immunoglobulin E (FcRI). A total of 40,759 transcripts derived from 11,300 genes were analyzed. Among the diverse genes that had not been previously associated with mast cells and that were constitutively expressed were those for the cytokine macrophage migration inhibitory factor neurohormone receptors such as growth hormone- releasing factor and melatonin and components of the exocytotic machinery. In addition, several dozen transcripts were differentially expressed in response to antigen-induced clustering of the FcRI. Included among these were the genes for preprorelaxin, mitogen-activated protein kinase kinase 3, and the dual specificity protein phosphatase, rVH6. Significantly, the majority of genes differentially expressed in this well-studied model of mast cell activation have not been identified before this analysis.

Key Words: receptor aggregation • signal transduction • exocytosis • cell differentiation • allergy

Abbreviations used: DTH, delayed-type hypersensitivity; GnRH, gonadotropin releasing hormone; MAPKK, mitogen-activated protein kinase kinase; MCP, monocyte chemotactic protein; MIF, macrophage migration inhibitory factor; PLA₂, phospholipase A₂; RT, reverse transcription; SAGE, serial analysis of gene expression; VAMP, vesicle- associated membrane protein.

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