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© The Rockefeller University Press, 0022-1007/1998/10/1465/ $5.00
The Journal of Experimental Medicine, Volume 188, Number 8, October 19, 1998 1465-1471

Articles

Assembly of Productive T Cell Receptor {delta} Variable Region Genes Exhibits Allelic Inclusion

Barry P. Sleckman, Bernard Khor, Robert Monroe, and Frederick W. Alt

From the Howard Hughes Medical Institute, Children's Hospital and Department of Genetics, Harvard Medical School and The Center for Blood Research, Boston, Massachusetts 02115

The generation of a productive "in-frame" T cell receptor β (TCR β), immunoglobulin (Ig) heavy (H) or Ig light (L) chain variable region gene can result in the cessation of rearrangement of the alternate allele, a process referred to as allelic exclusion. This process ensures that most {alpha}β T cells express a single TCR β chain and most B cells express single IgH and IgL chains. Assembly of TCR {alpha} and TCR {gamma} chain variable region genes exhibit allelic inclusion and {alpha}β and {gamma}{delta} T cells can express two TCR {alpha} or TCR {gamma} chains, respectively. However, it was not known whether assembly of TCR {delta} variable regions genes is regulated in the context of allelic exclusion. To address this issue, we have analyzed TCR {delta} rearrangements in a panel of mouse splenic {gamma}{delta} T cell hybridomas. We find that, similar to TCR {alpha} and {gamma} variable region genes, assembly of TCR {delta} variable region genes exhibits properties of allelic inclusion. These findings are discussed in the context of {gamma}{delta} T cell development and regulation of rearrangement of TCR {delta} genes.

Key Words: T cells • {gamma}{delta} T cells • T cell receptor rearrangement • allelic exclusion • T cell receptor {delta}

Address correspondence to Frederick W. Alt, Howard Hughes Medical Institute, Children's Hospital, Longwood Ave., Boston, MA 02115. Phone: 617-355-7290; Fax: 617-730-0432; E-mail: alt{at}rascal.med.harvard.edu

B.P. Sleckman's present address is Department of Pathology, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, MO 63110.

The first two authors contributed equally to this study.

Abbreviations used: BW, BW-1100.129.237; D, diversity; DN, double negative; DP, double positive; H, heavy; J, joining; L, light; RAG, recombinase activating gene; V, variable.


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