|
||
Articles |
B Inhibitors I
B
and I
Bβ

Department of Experimental Pathology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000
The transcription factor NF-
B is sequestered in the cytoplasm by the inhibitor proteins of the I
B family. Each member of the I
B exhibits structural and biochemical similarities as well as differences. In an effort to address the functional redundancy of two closely related I
B molecules, I
B
and I
Bβ, we generated knock-in mice by replacing the I
B
gene with the I
Bβ gene. The knock-in mice do not express I
B
, but express a T7-tagged I
Bβ under the promoter and regulatory sequence of ikba. Unlike the I
B
-deficient mice, which display severe postnatal developmental defects and die by postnatal day 8, homozygous knock-in mice survive to adulthood, are fertile, and exhibit no apparent abnormalities. Furthermore, thymocytes and embryonic fibroblasts from the knock-in animals exhibit an inducible NF-
B response similar to that of wild-type animals. These results indicate that I
B
and I
Bβ share significant similarities in their biochemical activity, and that they acquired their different functions from divergent expression patterns during evolution.
Key Words: nuclear factor
B I
B transgenic mice knockout mice hematopoiesis
Abbreviations used: EMSA, electrophoretic mobility shift assay; ES, embryonic stem; MEF, mouse embryo fibroblast, NF-
B, nuclear factor
B.
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Facebook
Reddit
Technorati
Twitter What's this?
| TABLE OF CONTENTS |
|