The Journal of Experimental Medicine
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A correction to this article has been published: J. Exp. Med. 188 (5) 1003
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© The Rockefeller University Press, 0022-1007/1998/8/465/ $5.00
The Journal of Experimental Medicine, Volume 188, Number 3, August 3, 1998 465-474

Articles

Hematopoietic Progenitor Cell Rolling in Bone Marrow Microvessels: Parallel Contributions by Endothelial Selectins and Vascular Cell Adhesion Molecule 1

Irina B. Mazo*, Jose-Carlos Gutierrez-Ramos||, Paul S. Frenette*,§, Richard O. Hynes, Denisa D. Wagner*,{ddagger}, and Ulrich H. von Andrian*,{ddagger}

From * The Center for Blood Research, the {ddagger} Department of Pathology, and the § Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; || Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

We have used intravital microscopy to study physiologically perfused microvessels in murine bone marrow (BM). BM sinusoids and venules, but not adjacent bone vessels, supported rolling interactions of hematopoietic progenitor cells. Rolling did not involve L-selectin, but was partially reduced in wild-type mice treated with antibodies to P- or E-selectin and in mice that were deficient in these two selectins. Selectin-independent rolling was mediated by {alpha}4 integrins, which interacted with endothelial vascular cell adhesion molecule (VCAM)-1. Parallel contribution of the endothelial selectins and VCAM-1 is not known to direct blood cell trafficking to other noninflamed tissues. This combination of constitutively expressed adhesion molecules may thus constitute a BM-specific recruitment pathway for progenitor cells analogous to the vascular addressins that direct selective lymphocyte homing to lymphoid organs.

Key Words: bone marrow • selectins • {alpha}4 integrin • intravital microscopy • homing

Address correspondence to Ulrich H. von Andrian, The Center for Blood Research, 200 Longwood Ave., Boston, MA 02115. Phone: 617-278-3130; Fax: 617-278-3190; E-mail: uva{at}cbr.med.harvard.edu

Abbreviations used: BM, bone marrow; BV, bone venule; CN, capillary network; CV, collecting venule; FDCP-mix, factor-dependent cells Paterson-mix; FL, fetal liver; F/P, fluorescein/protein; HPC, hematopoietic progenitor cells; IV, intermediate venule; PSV, postsinusoidal venule; VCAM, vascular cell adhesion molecule.


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