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J. Exp. Med.,
Volume 188, Number 2, July 20, 1998 365-372
By
From the * Department of Biotechnology, Faculty of Engineering, Okayama University,
Tsushima-Naka, Okayama 700-8530, Japan; the Mouse germinal center (GC) B cells have been shown to undergo secondary V(D)J (V, variable; D, diversity; J, joining) recombination (receptor editing) mediated by the reexpressed
products of recombination activating gene (RAG)-1 and RAG-2. We show here that interleukin (IL)-7 as well as IL-4 was effective in inducing functional RAG products in mouse IgD+ B
cells activated via CD40 in vitro. Blocking of the IL-7 receptor (IL-7R) by injecting an anti-
IL-7R monoclonal antibody resulted in a marked suppression of the reexpression of RAG-2
and subsequent V(D)J recombination in the draining lymph node of immunized mice, whereas
RAG-2 expression was not impaired in immunized IL-4-deficient mice. Further, these peripheral B cells activated in vitro or in vivo were found to express IL-7R. These findings indicate a novel role for IL-7 and IL-7R in inducing receptor editing in GC B cells.
Department of Biosciences, School of Science,
Kitasato University, Kanagawa 228, Japan; and the § Department of Molecular Genetics, Faculty of
Medicine, Kyoto University, Kyoto 606-8507, Japan
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