The Journal of Experimental Medicine
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 385K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hackam, D. J.
Right arrow Articles by Grinstein, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hackam, D. J.
Right arrow Articles by Grinstein, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/1998/7/351/ $5.00
The Journal of Experimental Medicine, Volume 188, Number 2, July 20, 1998 351-364

Articles

Host Resistance to Intracellular Infection: Mutation of Natural Resistance-associated Macrophage Protein 1 (Nramp1) Impairs Phagosomal Acidification

David J. Hackam*,{ddagger}, Ori D. Rotstein{ddagger}, Wei-jian Zhang*,{ddagger}, Samantha Gruenheid§, Philippe Gros§, and Sergio Grinstein*

From the * Division of Cell Biology, The Hospital for Sick Children, Toronto M5G 1X8, Ontario, Canada; the {ddagger} Department of Surgery, Toronto Hospital, University of Toronto, Toronto M5G 1X8, Ontario, Canada; and the § Department of Biochemistry, McGill University, Montreal, Quebec, Canada H3G 1Y6

The mechanisms underlying the survival of intracellular parasites such as mycobacteria in host macrophages remain poorly understood. In mice, mutations at the Nramp1 gene (for natural resistance-associated macrophage protein), cause susceptibility to mycobacterial infections. Nramp1 encodes an integral membrane protein that is recruited to the phagosome membrane in infected macrophages. In this study, we used microfluorescence ratio imaging of macrophages from wild-type and Nramp1 mutant mice to analyze the effect of loss of Nramp1 function on the properties of phagosomes containing inert particles or live mycobacteria. The pH of phagosomes containing live Mycobacterium bovis was significantly more acidic in Nramp1- expressing macrophages than in mutant cells (pH 5.5 ± 0.06 versus pH 6.6 ± 0.05, respectively; P <0.005). The enhanced acidification could not be accounted for by differences in proton consumption during dismutation of superoxide, phagosomal buffering power, counterion conductance, or in the rate of proton "leak", as these were found to be comparable in wild-type and Nramp1-deficient macrophages. Rather, after ingestion of live mycobacteria, Nramp1-expressing cells exhibited increased concanamycin-sensitive H+ pumping across the phagosomal membrane. This was associated with an enhanced ability of phagosomes to fuse with vacuolar-type ATPase–containing late endosomes and/or lysosomes. This effect was restricted to live M. bovis and was not seen in phagosomes containing dead M. bovis or latex beads. These data support the notion that Nramp1 affects intracellular mycobacterial replication by modulating phagosomal pH, suggesting that Nramp1 plays a central role in this process.

Key Words: mycobacterium tuberculosis • phagosome • phagocytosis • macrophage • proton pump

Address correspondence to Sergio Grinstein, Division of Cell Biology, Hospital for Sick Children, 555 University Ave., Toronto, M5G 1X8, Ontario, Canada. Phone: 416-813-5727; Fax: 416-813-5028; E-mail: sga{at}sickkids.on.ca

This research was funded by operating grants awarded to P. Gros from the National Institute of Allergy and Infectious Diseases (RO1 AI-35247-0352) and to S. Grinstein and O.D. Rotstein by the Medical Research Council of Canada and the National Sanatorium Association. S. Grinstein is cross-appointed to the Department of Biochemistry of the University of Toronto. P. Gros and S. Grinstein are International Scholars of the Howard Hughes Medical Institute. D.J. Hackam is the recipient of an Ethicon-Society of University Surgeons Surgical Research Award and a Medical Research Council of Canada Fellowship.

Abbreviations used: BCG, Mycobacterium bovis strain bacillus Calmette-Guérin, substrain Montreal; CCCP, carbonyl cyanide-m-chlorophenyl hydrazone; LAMP, lysosomal-associated membrane protein; NHS-CF, N-hydroxysuccinimidyl 5-(and 6-)-carboxyfluorescein; Nramp, natural resistance-associated macrophage protein; pHe, endosomal pH; pHp, phagosomal pH; SLO, streptolysin O; V-ATPases, vacuolar-type proton ATPases.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS