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Horst Maxeiner^*, Jens Husemann^*, Christian A. Thomas^*,, John D. Loike^*, Joseph El Khoury^,*, and Samuel C. Silverstein^*,

From the ^* Department of Physiology and Cellular Biophysics, and Department of Medicine, Columbia University College of Physicians and Surgeons, New York 10032; and the Department of Medicine, Beth Israel Hospital, Albert Einstein College of Medicine, New York 10003

Oxidized low-density lipoprotein (oxLDL) is considered one of the principal effectors of atherogenesis. To explore mechanisms by which oxLDL affects human mononuclear phagocytes, we incubated these cells in medium containing oxLDL, acetylated LDL (acLDL), or native LDL, or on surfaces coated with these native and modified lipoproteins. The presence of soluble oxLDL, acLDL, or native LDL in the medium did not stimulate H₂O₂ secretion by macrophages. In contrast, macrophages adherent to surfaces coated with oxLDL secreted three- to fourfold more H₂O₂ than macrophages adherent to surfaces coated with acLDL or native LDL. Freshly isolated blood monocytes secreted little H₂O₂ regardless of the substrate on which they were plated. H₂O₂ secretion was maximal in cells maintained for 4–6 d in culture before plating on oxLDL-coated surfaces. Fucoidan, a known ligand of class A macrophage scavenger receptors (MSR-A), significantly reduced macrophage adhesion to surfaces coated with oxLDL or acLDL. Monoclonal antibody SMO, which blocks oxLDL binding to CD36, did not inhibit adhesion of macrophages to oxLDL-coated surfaces but markedly reduced H₂O₂ secretion by these cells. These studies show that MSR-A is primarily responsible for adhesion of macrophages to oxLDL-coated surfaces, that CD36 signals H₂O₂ secretion by macrophages adherent to these surfaces, and that substrate-bound, but not soluble, oxLDL stimulates H₂O₂ secretion by macrophages.

Key Words: macrophages • scavenger receptors • CD36 • oxidized low-density lipoproteins • H₂O₂ secretion

Abbreviations used: acLDL, acetylated low-density lipoprotein; DiI, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate; HRP, horseradish peroxidase; KRBG-A, Krebs Ringer buffer with glucose and albumin; MSR, macrophage scavenger receptors; oxLDL, oxidized low-density lipoprotein.

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