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J. Exp. Med.,
Volume 188, Number 12, December 21, 1998 2225-2231
1,3-fucosyltransferase VII Gene Expression
By
From the * Department of Microbiology-Immunology and the The Departments of Biomedical Engineering
and Anesthesiology, Northwestern Medical School, Chicago, Illinois 60611; and the § Department of
Pathology, University of Michigan, Ann Arbor, Michigan 48109
1,3-fucosyltransferase, FucT-VII, is crucial for the formation of ligands for all three selectins, and its expression regulates the synthesis of these ligands. Short-term polarized T helper
(Th)1, but not Th2 or naive CD4+ T cells, can home to sites of inflammation, but the molecular basis for this difference has remained unclear. Here we show that naive CD4+ T cells do
not express FucT-VII and fail to bind vascular selectins. We also show that when CD4+ T cells
are activated in the presence of the Th1 polarizing cytokine interleukin (IL)-12, levels of
FucT-VII mRNA and binding to E- and P-selectin are significantly augmented. In contrast,
activation of CD4+ T cells in the presence of IL-4, a Th2 polarizing cytokine, inhibited FucT-VII expression and binding to vascular selectins. T cell activation upregulated expression of the
Core2 transferase, C2GnT, equivalently regardless of the presence or absence of polarizing cytokines. These data indicate that the selective ability of Th1 cells, as opposed to Th2 cells or
naive CD4+ T cells, to recognize vascular selectins and home to sites of inflammation is controlled principally by the expression of a single gene, FucT-VII.
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