The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1998/12/2187/ $5.00
The Journal of Experimental Medicine, Volume 188, Number 11, December 7, 1998 2187-2191

Brief Definitive Reports

{alpha}dβ2 Integrin Is Expressed on Human Eosinophils and Functions as an Alternative Ligand for Vascular Cell Adhesion Molecule 1 (VCAM-1)

Mitchell H. Grayson*, Monica Van der Vieren{ddagger}, Sherry A. Sterbinsky*, W. Michael Gallatin{ddagger}, Patricia A. Hoffman{ddagger}, Donald E. Staunton{ddagger}, and Bruce S. Bochner*

From * The Johns Hopkins University School of Medicine, Division of Clinical Immunology, Baltimore, Maryland 21224; and {ddagger} ICOS Corporation, Bothell, Washington 98021

The β2 family of integrins, CD11a, CD11b, CD11c, and {alpha}d, are expressed on most leukocytes. We show that the newest member of this family, {alpha}d, is expressed on human eosinophils in peripheral blood, and surface expression can be upregulated within minutes by phorbol ester or calcium ionophore A23187. Culture of eosinophils with interleukin 5 (IL-5) leads to a two- to fourfold increase in {alpha}d levels by 3–7 d without a change in {alpha}4 integrin expression. Eosinophils isolated from late phase bronchoalveolar lavage fluids express {alpha}d at levels similar to that seen after 3 d of IL-5 culture. Regarding {alpha}dβ2 ligands, in both freshly isolated and IL-5–cultured eosinophils, as well as {alpha}dβ2-transfected Chinese hamster ovary cells, {alpha}dβ2 can function as a ligand for vascular cell adhesion molecule 1 (VCAM-1). This conclusion is based on the ability of monoclonal antibodies to {alpha}d, β2, or VCAM-1 to block cell attachment in static adhesion assays. In experiments with eosinophils, the relative contribution of {alpha}dβ2 integrin– mediated adhesion is enhanced after IL-5 culture. These experiments demonstrate that {alpha}dβ2 is an alternative ligand for VCAM-1, and this integrin may play a role in eosinophil adhesion to VCAM-1 in states of chronic inflammation.

Key Words: β2 integrin • eosinophil • adhesion • vascular cell adhesion molecule 1

Address correspondence to Bruce S. Bochner, Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224. Phone: 410-550-2131; Fax: 410-550-2130; E-mail: bbochner{at}welchlink.welch.jhu.edu


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