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J. Exp. Med.,
Volume 188, Number 11, December 7, 1998 2187-2191
d
2 Integrin Is Expressed on Human Eosinophils
and Functions as an Alternative Ligand for
Vascular Cell Adhesion Molecule 1 (VCAM-1)
By
From * The Johns Hopkins University School of Medicine, Division of Clinical Immunology,
Baltimore, Maryland 21224; and The ICOS Corporation, Bothell, Washington 98021
2 family of integrins, CD11a, CD11b, CD11c, and 
d, are expressed on most leukocytes. We show that the newest member of this family, d, is expressed on human eosinophils
in peripheral blood, and surface expression can be upregulated within minutes by phorbol ester
or calcium ionophore A23187. Culture of eosinophils with interleukin 5 (IL-5) leads to a two-
to fourfold increase in d levels by 3-7 d without a change in 4 integrin expression. Eosinophils isolated from late phase bronchoalveolar lavage fluids express d at levels similar to that
seen after 3 d of IL-5 culture. Regarding d2 ligands, in both freshly isolated and IL-5-cultured eosinophils, as well as d2-transfected Chinese hamster ovary cells, d2 can function
as a ligand for vascular cell adhesion molecule 1 (VCAM-1). This conclusion is based on the
ability of monoclonal antibodies to d, 2, or VCAM-1 to block cell attachment in static adhesion assays. In experiments with eosinophils, the relative contribution of d2 integrin-
mediated adhesion is enhanced after IL-5 culture. These experiments demonstrate that d2 is an alternative ligand for VCAM-1, and this integrin may play a role in eosinophil adhesion to
VCAM-1 in states of chronic inflammation.
2 integrin;
eosinophil;
adhesion;
vascular cell adhesion molecule 1
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