The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1998/12/2083/ $5.00
The Journal of Experimental Medicine, Volume 188, Number 11, December 7, 1998 2083-2090

Articles

2B4, the Natural Killer and T Cell Immunoglobulin Superfamily Surface Protein, Is a Ligand for CD48

Marion H. Brown*, Kent Boles{ddagger}, P. Anton van der Merwe*, Vinay Kumar§, Porunelloor A. Mathew{ddagger}, and A. Neil Barclay*

From the * Medical Research Council Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; the {ddagger} Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, Texas 76107; and the § Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas 75235

2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin-coated fluorescent beads bound to rodent cells, and binding was completely blocked by CD48 monoclonal antibodies (mAbs). Using surface plasmon resonance, we showed that purified soluble mCD48 bound m2B4 with a six- to ninefold higher affinity (Kd {approx} 16 µM at 37°C) than its other ligand, CD2. Human CD48 bound human 2B4 with a similar affinity (Kd {approx} 8 µM). The finding of an additional ligand for CD48 provides an explanation for distinct functional effects observed on perturbing CD2 and CD48 with mAbs or by genetic manipulation.

Key Words: 2B4 • CD48 • natural killer cell • T cell • ligand

Address correspondence to Marion H. Brown, MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, South Parks Rd., Oxford, OX1 3RE, UK. Phone: 01-865-275-594; Fax: 01-865-275-591; E-mail: marion.brown{at}path.ox.ac.uk

The research was supported by the Medical Research Council and the European Union Biotechnology Programme.

Abbreviations used: CD4d3+4, domains 3 and 4 of rat CD4; CD4L, rat CD4 leader; h, human; IgSF, immunoglobulin superfamily; m, mouse; r, rat; RU, response unit(s).


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