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© The Rockefeller University Press, 0022-1007/1998/7/61/ $5.00
The Journal of Experimental Medicine, Volume 188, Number 1, July 1, 1998 61-70

Articles

Evolution of a Complex T Cell Receptor Repertoire during Primary and Recall Bacterial Infection

Dirk H. Busch, Ingrid Pilip, and Eric G. Pamer

From the Section of Infectious Diseases and the Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520

The mechanisms underlying the genesis and maintenance of T cell memory remain unclear. In this study, we examined the evolution of a complex, antigen-specific T cell population during the transition from primary effector to memory T cells after Listeria monocytogenes infection. T cell populations specific for listeriolysin O (LLO)91–99, the immunodominant epitope recognized by H2-Kd–restricted T lymphocytes, were directly identified in immune spleens using tetrameric H2-Kd–epitope complexes. The T cell receptor (TCR) Vβ repertoire of specific T cells was determined by direct, ex vivo staining with a panel of mAbs. We demonstrate that LLO91–99-specific, primary effector T cell populations have a diverse TCR Vβ repertoire. Analyses of memory T cell populations demonstrated similar TCR diversity. Furthermore, experiments with individual mice demonstrated that primary effector and memory T cells have indistinguishable TCR repertoires. Remarkably, after reinfection with L. monocytogenes, LLO91–99-specific T cells have a narrower TCR repertoire than do primary effector or memory T cells. Thus, our studies show that the TCR repertoire of primary effector T lymphocytes is uniformly transmitted to memory T cells, whereas expansion of memory T cells is selective.

Key Words: T cell receptor repertoire • cytotoxic T lymphocytes • Listeria monocytogenes • effector/memory T cells • recall

Address correspondence to Eric G. Pamer, Sections of Infectious Diseases and Immunobiology, Yale University, New Haven, CT 06520. Phone: 203-785-3561; Fax: 203-785-3864; E-mail: eric.pamer{at}yale.edu

Abbreviations used: β2m, β2 microglobulin; BirA, biotin operon repressor protein A; LLO, listeriolysin O; SB, staining buffer.


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