The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1998/5/1543/ $5.00
The Journal of Experimental Medicine, Volume 187, Number 9, May 4, 1998 1543-1548

Brief Definitive Reports

Vaccination with DNA Encoding an Immunodominant Myelin Basic Protein Peptide Targeted to Fc of Immunoglobulin G Suppresses Experimental Autoimmune Encephalomyelitis

Anna Lobell*, Robert Weissert{ddagger}, Maria K. Storch§, Cecilia Svanholm*, Katrien L. de Graaf{ddagger}, Hans Lassmann§, Roland Andersson*, Tomas Olsson{ddagger}, and Hans Wigzell*

From the * Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the {ddagger} Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the § Neurological Institute, University of Vienna, A-1090 Vienna, Austria

We explore here if vaccination with DNA encoding an autoantigenic peptide can suppress autoimmune disease. For this purpose we used experimental autoimmune encephalomyelitis (EAE), which is an autoaggressive disease in the central nervous system and an animal model for multiple sclerosis. Lewis rats were vaccinated with DNA encoding an encephalitogenic T cell epitope, guinea pig myelin basic protein peptide 68–85 (MBP68–85), before induction of EAE with MBP68–85 in complete Freund's adjuvant. Compared to vaccination with a control DNA construct, the vaccination suppressed clinical and histopathological signs of EAE, and reduced the interferon {gamma} production after challenge with MBP68–85. Targeting of the gene product to Fc of IgG was essential for this effect. There were no signs of a Th2 cytokine bias. Our data suggest that DNA vaccines encoding autoantigenic peptides may be useful tools in controlling autoimmune disease.

Address correspondence to Anna Lobell, Pharmacia & Upjohn, P12:4, 112 87 Stockholm, Sweden. Phone: 46-8-6958303; Fax: 46-8-6185882; E-mail: anna.lobell{at}eu.pnu.com


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