The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1998/4/1157/ $5.00
The Journal of Experimental Medicine, Volume 187, Number 7, April 6, 1998 1157-1161

Brief Definitive Reports

Molecular Cloning of the cDNA Encoding pp36, a Tyrosine-phosphorylated Adaptor Protein Selectively Expressed by T Cells and Natural Killer Cells

John R. Weber*, Sigurd Ørstavik{ddagger}, Knut Martin Torgersen§, Niels Christian Danbolt§, Siri F. Berg§, James C. Ryan*,||, Kjetil Taskén{ddagger}, John B. Imboden*, and John T. Vaage§

From the * Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the {ddagger} Institute of Medical Biochemistry and § Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the || Veterans Administration Medical Center, University of California, San Francisco, California 94122

Activation of T and natural killer (NK) cells leads to the tyrosine phosphorylation of pp36 and to its association with several signaling molecules, including phospholipase C{gamma}-1 and Grb2. Microsequencing of peptides derived from purified rat pp36 protein led to the cloning, in rat and man, of cDNA encoding a T- and NK cell–specific protein with several putative Src homology 2 domain–binding motifs. A rabbit antiserum directed against a peptide sequence from the cloned rat molecule recognized tyrosine phosphorylated pp36 from pervanadate-treated rat thymocytes. When expressed in 293T human fibroblast cells and tyrosine-phosphorylated, pp36 associated with phospholipase C{gamma}-1 and Grb2. Studies with GST–Grb2 fusion proteins demonstrated that the association was specific for the Src homology 2 domain of Grb-2. Molecular cloning of the gene encoding pp36 should facilitate studies examining the role of this adaptor protein in proximal signaling events during T and NK cell activation.

Address correspondence to John B. Imboden, Box 0868, Department of Medicine, University of California, San Francisco, CA 94143. Phone: 415-206-6641; Fax: 415-648-8425; E-mail: imboden{at}itsa.ucsf.edu

Note added in proof. Zhang et al. recently reported the cloning of human and mouse pp36. Cell. 92:83.


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