The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 120K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohteki, T.
Right arrow Articles by Ohashi, P. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohteki, T.
Right arrow Articles by Ohashi, P. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/1998/3/967/ $5.00
The Journal of Experimental Medicine, Volume 187, Number 6, March 16, 1998 967-972

Brief Definitive Reports

The Transcription Factor Interferon Regulatory Factor 1 (IRF-1) Is Important during the Maturation of Natural Killer 1.1+ T Cell Receptor–{alpha}/β+ (NK1+ T) Cells, Natural Killer Cells, and Intestinal Intraepithelial T Cells

Toshiaki Ohteki*, Hiroki Yoshida{ddagger}, Toshifumi Matsuyama{ddagger}, Gordon S. Duncan{ddagger}, Tak W. Mak*,{ddagger}, and Pamela S. Ohashi*

From the * Ontario Cancer Institute, Departments of Medical Biophysics and Immunology, Toronto, Ontario, Canada, M5G 2M9; and the {ddagger} Amgen Institute, Toronto, Ontario, Canada, M5G 2C1

In contrast to conventional T cells, natural killer (NK) 1.1+ T cell receptor (TCR)-{alpha}/β+ (NK1+T) cells, NK cells, and intestinal intraepithelial lymphocytes (IELs) bearing CD8-{alpha}/{alpha} chains constitutively express the interleukin (IL)-2 receptor (R)β/15Rβ chain. Recent studies have indicated that IL-2Rβ/15Rβ chain is required for the development of these lymphocyte subsets, outlining the importance of IL-15. In this study, we investigated the development of these lymphocyte subsets in interferon regulatory factor 1–deficient (IRF-1–/–) mice. Surprisingly, all of these lymphocyte subsets were severely reduced in IRF-1–/– mice. Within CD8-{alpha}/{alpha}+ intestinal IEL subset, TCR-{gamma}/{delta}+ cells and TCR-{alpha}/β+ cells were equally affected by IRF gene disruption. In contrast to intestinal TCR-{gamma}/{delta}+ cells, thymic TCR-{gamma}/{delta}+ cells developed normally in IRF-1–/– mice. Northern blot analysis further revealed that the induction of IL-15 messenger RNA was impaired in IRF-1–/– bone marrow cells, and the recovery of these lymphocyte subsets was observed when IRF-1–/– cells were cultured with IL-15 in vitro. These data indicate that IRF-1 regulates IL-15 gene expression, which may control the development of NK1+T cells, NK cells, and CD8-{alpha}/{alpha}+ IELs.

Address correspondence to Toshiaki Ohteki, Ontario Cancer Institute, Departments of Medical Biophysics and Immunology, 610 University Ave., Toronto, Ontario, Canada, M5G 2M9. Phone: 416-946-2000; Fax: 416-946-2086; E-mail: tohteki{at}oci.utoronto.ca


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS