The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1998/3/721/ $5.00
The Journal of Experimental Medicine, Volume 187, Number 5, March 2, 1998 721-731

Articles

Aerosol-induced Immunoglobulin (Ig)-E Unresponsiveness to Ovalbumin Does Not Require CD8+ or T Cell Receptor (TCR)-{gamma}/{delta}+ T Cells or Interferon (IFN)-{gamma} in a Murine Model of Allergen Sensitization

Brian W.P. Seymour*,{ddagger}, Laurel J. Gershwin{ddagger}, and Robert L. Coffman*

From the * Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304; and the {ddagger} Department of Pathology, Microbiology, and Immunology, University of California, School of Veterinary Medicine, Davis, California 95616

Mice exposed for 20 min daily to aerosolized ovalbumin (OVA) for 10 d at concentrations from 1 to 0.01% OVA made greatly reduced immunoglobulin (Ig)-E responses to subsequent immunogenic OVA challenges, given either intraperitoneally or by aerosol. This IgE-specific unresponsiveness lasted for at least four months. However, these aerosol-treated mice were primed for larger OVA-specific IgG1 and IgG2a responses. The specific reduction in IgE responses was not due to preferential induction of a T helper (Th)-1 response as aerosol OVA– primed mice made greatly reduced Th2 and no detectable Th1 response after rechallenge in vitro. Consistent with this, the increase in circulating eosinophils observed in control Th2-primed mice was absent in aerosol OVA–treated animals. Interferon (IFN)-{gamma} was not required for this unresponsiveness, as IFN-{gamma} knockout mice and anti–IFN-{gamma} antibody-treated wild-type mice had greatly reduced levels of IgE similar to wild-type controls. CD8+ T cells played a relatively small role as IgE responses were reduced to about the same extent in β2 microglobulin-deficient, or in anti-CD8–treated wild-type mice as in normal mice after aerosol OVA treatment. Similarly, T cell receptor (TCR)-{gamma}/{delta} T cells were not required for maximal inhibition of the IgE response. These results demonstrate that exposure to inhaled protein antigens can induce a state of unresponsiveness of CD4+ T cells that results in a prolonged loss of IgE and eosinophil responses to subsequent challenges. This T cell unresponsiveness was shown not to require CD8+ or TCR-{gamma}/{delta}+ T cells or IFN-{gamma}.

1 Abbreviations used in this paper: AL, aluminum hydroxide; β2m–/–, β2 microglobulin knock-out mice; DIG, digoxigenin; IFN-{gamma}–/–, IFN-{gamma} gene knock-out mice; IP, intraperitoneally; NIP, nitroiodophenyl; T, Tween 20; TCR-{delta}–/–, T cell receptor {delta} knock-out mice.

1 Abbreviations used in this paper: AL, aluminum hydroxide; β2m–/–, β2 microglobulin knock-out mice; DIG, digoxigenin; IFN-{gamma}–/–, IFN-{gamma} gene knock-out mice; IP, intraperitoneally; NIP, nitroiodophenyl; T, Tween 20; TCR-{delta}–/–, T cell receptor {delta} knock-out mice.

We acknowledge Dr. Gabriele Grunig for technical assistance and expert advice in the preparation of this manuscript and Drs. Amy Beebe, Stephen Hurst, Douglas Robinson, and Thierry von der Weid for constructive comments on the manuscript. We also thank Dr. Derry Roopenian of The Jackson Laboratories for the original breeding pairs of BALB/c β2m–/– mice.

This research was supported in part by the University of California, Tobacco Related Disease Research Program. The DNAX Research Institute is supported by the Schering Plough Corporation.

Address correspondence to R.L. Coffman, Ph.D., Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Avenue, Palo Alto, CA 94304. Phone: 650-496-1261; Fax: 650-496-1200; E-mail: coffman{at}dnax.org


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