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© The Rockefeller University Press, 0022-1007/1998/3/703/ $5.00
The Journal of Experimental Medicine, Volume 187, Number 5, March 2, 1998 703-709

Articles

Regulation of B Lymphocyte Development by the Truncated Immunoglobulin Heavy Chain Protein Dµ

Ulla-Carin Tornberg*, Ingela Bergqvist*, Matthias Haury{ddagger}, and Dan Holmberg*,{ddagger}

From the * Department of Cell and Molecular Biology, Umeå University, S-901 87 Umeå, Sweden; and the {ddagger} Department of Immunology, Institut Pasteur, 750 15 Paris, Cedex 15, France

The development of B lymphocytes from progenitor cells is dependent on the expression of a pre–B cell–specific receptor made up by a µ heavy chain associated with the surrogate light chains, immunoglobulin (Ig){alpha}, and Igβ. A variant pre–B cell receptor can be formed in which the µ heavy chain is exchanged for a truncated µ chain denoted Dµ. To investigate the role of this receptor in the development of B cells, we have generated transgenic mice that express the Dµ protein in cells of the B lineage. Analysis of these mice reveal that Dµ expression leads to a partial block in B cell development at the early pre–B cell stage, probably by inhibiting VH to DHJH rearrangement. Furthermore, we provide evidence that Dµ induces VL to JL rearrangements.

We thank Drs. A. Cumano, J. Demengeot, B. Eriksson, and P. Perreira for discussions and for reviewing the manuscript.

This work was supported by a grant from the Swedish Natural Science Research Council.

Address correspondence to Dan Holmberg, Department of Immunology, Institut Pasteur, 25 rue de Dr. Roux, 750 15 Paris Cedex 15, France. Phone: 33-1-4568-8543 Fax: 33-1-4568-8921; E-mail: holmberg{at}pasteur.fr

1 Abbreviations used in this paper: HSA, heat stable antigen; pBCR, pre–B cell receptor; RF, reading frame.

I. Bergqvist and U.-C. Tornberg contributed equally to this work.


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