The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1998/2/525/ $5.00
The Journal of Experimental Medicine, Volume 187, Number 4, February 16, 1998 525-536

Articles

Subsets of Transgenic T Cells That Recognize CD1 Induce or Prevent Murine Lupus: Role of Cytokines

Defu Zeng*, Michael Dick*, Lirong Cheng*, Masahiko Amano*, Sussan Dejbakhsh-Jones*, Philip Huie{ddagger}, Richard Sibley{ddagger}, and Samuel Strober*

From the Division of Immunology and Rheumatology, * Department of Medicine, and {ddagger} Department of Pathology, Stanford University School of Medicine, Stanford, California 94305-5111

T cells with T cell receptor (TCR) transgenes that recognized CD1 on syngeneic B cells stimulated B cells to secrete immunoglobulins in vitro. The CD4+, CD8+, or CD4–CD8– T cells from the spleen of the TCR transgenic BALB/c donors induced lupus with anti–double stranded DNA antibodies, proteinuria, and immune complex glomerulonephritis in irradiated BALB/c nude mice reconstituted with nude bone marrow. Injection of purified CD4–CD8– T cells from the marrow of transgenic donors prevented the induction of lupus by the transgenic T cells. Transgenic T cells that induced lupus secreted large amounts of interferon (IFN)-{gamma} and little interleukin (IL)-4, and those that prevented lupus secreted large amounts of IL-4 and little IFN-{gamma} or IL-10.

This work was supported by a grant from The American Lupus Society, a research grant from the Arthritis Foundation, and a National Institutes of Health grant AI-40093. D. Zeng was supported in part by a fellowship from Activated Cell Therapy, Inc. We thank Drs. G. Rolink and B. Kotzin for advice concerning measurement of serum immunoglobulins, A. Mukhopadhyay for technical assistance, and V. Cleaver for preparation of the manuscript.

Address correspondence to Samuel Strober, Division of Immunology and Rheumatology, Rm. S105B, Stanford University School of Medicine, 300 Pasteur Dr., Stanford, CA 94305-5111. Phone: 650-723-6500; Fax: 650-725-6104; E-mail: sstrober{at}leland.stanford.edu

1 Abbreviations used in this paper: BCL, B cell lymphoma; BM, bone marrow; cGy, centiGray; DN, double-negative; ds, double-stranded; SP, single-positive.


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