J. Exp. Med., Volume 187, Number 2, January 19, 1998 253-258

BRIEF DEFINITIVE REPORT:
Highly Biased CDR3 Usage in Restricted Sets of  Chain Variable Regions During Viral Superantigen 9 Response

By Cristina Ciurli,^* David N. Posnett,^§ Rafick-Pierre Sékaly,^*¶ and François Denis^*

From the ^* Laboratoire d'Immunologie, Institut de Recherches Cliniques de Montréal, Montréal (QC), Canada, H2W 1R7;  Département de Microbiologie-Immunologie, Faculté de Médecine, Université de Montréal, Montréal (QC), Canada, H3C 3J7; ^§ Department of Medicine, and  Immunology Program, Graduate School of Medical Sciences, Cornell University Medical College, New York 10021; and ^¶ Department of Microbiology and Immunology, School of Medicine, McGill University, Montréal (QC), Canada, H3A 2B4

Superantigens encoded by the mouse mammary tumor virus can stimulate a large proportion of T cells through interaction with germline-encoded regions of the T cell receptor  chain like the hypervariable region 4 (HV4) loop. However, several lines of evidence suggest that somatically generated determinants in the CDR3 region might influence superantigen responses. We stimulated T cells from donors differing at the BV6S7 allele with vSAG9 to assess the nature and structure of the T cell receptor in amplified T cells and to evaluate the contribution of non-HV4 elements in vSAG recognition. This report demonstrates that vSAG9 stimulation caused the expansion of TCR BV6-expressing T cells, although to varying degrees depending on the BV6 subfamily. The BV6S7 subfamily was preferentially expanded in all donors, but in donors homozygous for the BV6S7*2 allele, a significant number of BV6S5 T cells were amplified and showed a highly biased  chain junctional region (BJ) and CDR3 usage. As CDR3 regions are involved in major histocompatibility complex (MHC)-peptide interaction, such a selection is highly suggestive of an intimate MHC-TCR interaction and would imply that the topology of the MHC-vSAG-TCR complex is similar to the one occurring during conventional antigen recognition.

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