The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1998/1/245/ $5.00
The Journal of Experimental Medicine, Volume 187, Number 2, January 19, 1998 245-251

Articles

Invariant Chain–independent Function of H-2M in the Formation of Endogenous Peptide–Major Histocompatibility Complex Class II Complexes In Vivo

Susan Kovats{ddagger}, Catherine E. Grubin{ddagger}, Susan Eastman*,{ddagger}, Paul deRoos*,{ddagger}, Ashok Dongre*,{ddagger}, Luc Van Kaer*,§, and Alexander Y. Rudensky*,{ddagger}

From the * Howard Hughes Medical Institute and the {ddagger} Department of Immunology, University of Washington School of Medicine, Seattle, Washington 98195; and the § Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

Efficient loading of major histocompatibility complex class II molecules with peptides requires the invariant chain (Ii) and the class II–like molecule H-2M. Recent in vitro biochemical studies suggest that H2-M may function as a chaperone to rescue empty class II dimers. To test this hypothesis in vivo, we generated mice lacking both Ii and H-2M (Ii–/–M–/–). Antigen presenting cells (APCs) from Ii–/–M–/– mice, as compared with APCs from Ii–/– mice, exhibit a significant reduction in their ability to present self-peptides to a panel of class II I-Ab–restricted T cells. As a consequence of this defect in the loading of self peptides, CD4+ thymocyte development is profoundly impaired in Ii–/–M–/– mice, resulting in a peripheral CD4+ T cell population with low levels of T cell receptor expression. These findings are consistent with the idea that H-2M functions as a chaperone in the peptide loading of class II molecules in vivo.

Address correspondence to Dr. Alexander Rudensky, Howard Hughes Medical Institute, University of Washington, Box 357370, Seattle, WA 98195. Phone: 206-685-7644; Fax: 206-685-3612; E-mail: sasha{at}nucleus.immunol.washington.edu

1 Abbreviations used in this paper: AAT, aspartate aminotransferase; CLIP, Class II associated invariant chain peptide; Ii, invariant chain; LDLr, LDL receptor; M, H-2M.


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