J. Exp. Med., Volume 187, Number 2, January 19, 1998 205-216

Molecular Mechanisms of Lymphocyte Homing to Peripheral Lymph Nodes

By R. Aaron Warnock,^* Sanaz Askari, Eugene C. Butcher,^* and Ulrich H. von Andrian

From the ^* Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and the Veterans Affairs Palo Alto Health Care Systems, Palo Alto, California 94304; and  The Center for Blood Research and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115

To characterize the adhesion cascade that directs lymphocyte homing to peripheral lymph nodes (PLNs), we investigated the molecular mechanisms of lymphocyte interactions with the microvasculature of subiliac lymph nodes. We found that endogenous white blood cells and adoptively transferred lymph node lymphocytes (LNCs) tethered and rolled in postcapillary high endothelial venules (HEVs) and to a lesser extent in collecting venules. Similarly, firm arrest occurred nearly exclusively in the paracortical HEVs. Endogenous polymorphonuclear (PMNs) and mononuclear leukocytes (MNLs) attached and rolled in HEVs at similar frequencies, but only MNLs arrested suggesting that the events downstream of primary rolling interactions critically determine the specificity of lymphocyte recruitment. Antibody inhibition studies revealed that L-selectin was responsible for attachment and rolling of LNCs, and that LFA-1 was essential for sticking. LFA-1-dependent arrest was also abolished by pertussis toxin, implicating a requirement for G_i-protein-linked signaling. 4 integrins, which play a critical role in lymphocyte homing to Peyer's Patches, made no significant contribution to attachment, rolling, or sticking in resting PLNs. Velocity analysis of interacting LNCs revealed no detectable contribution by LFA-1 to rolling. Taken together, our results suggest that lymphocyte- HEV interactions within PLNs are almost exclusively initiated by L-selectin followed by a G protein-coupled lymphocyte-specific activation event and activation-induced engagement of LFA-1. These events constitute a unique adhesion cascade that dictates the specificity of lymphocyte homing to PLNs.

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• Okada, T., Ngo, V. N., Ekland, E. H., Forster, R., Lipp, M., Littman, D. R., Cyster, J. G. (2002). Chemokine Requirements for B Cell Entry to Lymph Nodes and Peyer's Patches. JEM 196: 65-75 [Abstract] [Full Text]  
• Grabovsky, V., Dwir, O., Alon, R. (2002). Endothelial Chemokines Destabilize L-selectin-mediated Lymphocyte Rolling without Inducing Selectin Shedding. J. Biol. Chem. 277: 20640-20650 [Abstract] [Full Text]  
• Piccio, L., Rossi, B., Scarpini, E., Laudanna, C., Giagulli, C., Issekutz, A. C., Vestweber, D., Butcher, E. C., Constantin, G. (2002). Molecular Mechanisms Involved in Lymphocyte Recruitment in Inflamed Brain Microvessels: Critical Roles for P-Selectin Glycoprotein Ligand-1 and Heterotrimeric Gi-Linked Receptors. J. Immunol. 168: 1940-1949 [Abstract] [Full Text]  
• Hokari, R., Miura, S., Nagata, H., Fujimori, H., Koseki, S., Kato, S., Kurose, I., Sekizuka, E., Granger, D. N., Ishii, H. (2001). Intercellular cell adhesion molecule-1 regulates lymphocyte movement into intestinal microlymphatics of rat Peyer's patches. J. Leukoc. Biol. 70: 896-902 [Abstract] [Full Text]  
• Kennedy, N. J., Russell, J. Q., Michail, N., Budd, R. C. (2001). Liver Damage by Infiltrating CD8+ T Cells Is Fas Dependent. J. Immunol. 167: 6654-6662 [Abstract] [Full Text]  
• Palframan, R. T., Jung, S., Cheng, G., Weninger, W., Luo, Y., Dorf, M., Littman, D. R., Rollins, B. J., Zweerink, H., Rot, A., von Andrian, U. H. (2001). Inflammatory Chemokine Transport and Presentation in HEV: A Remote Control Mechanism for Monocyte Recruitment to Lymph Nodes in Inflamed Tissues. JEM 194: 1361-1374 [Abstract] [Full Text]  
• Abraham, C., Miller, J. (2001). Molecular Mechanisms of IL-2 Gene Regulation Following Costimulation Through LFA-1. J. Immunol. 167: 5193-5201 [Abstract] [Full Text]  
• Weninger, W., Crowley, M. A., Manjunath, N., von Andrian, U. H. (2001). Migratory Properties of Naive, Effector, and Memory CD8+ T Cells. JEM 194: 953-966 [Abstract] [Full Text]  
• Fors, B. P., Goodarzi, K., von Andrian, U. H. (2001). L-Selectin Shedding Is Independent of Its Subsurface Structures and Topographic Distribution. J. Immunol. 167: 3642-3651 [Abstract] [Full Text]  
• Koseki, S., Miura, S., Fujimori, H., Hokari, R., Komoto, S., Hara, Y., Ogino, T., Nagata, H., Goto, M., Hachimura, S., Kaminogawa, S., Ishii, H. (2001). In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine. Int Immunol 13: 1165-1174 [Abstract] [Full Text]  
• Mikulowska-Mennis, A., Xu, B., Berberian, J. M., Michie, S. A. (2001). Lymphocyte Migration to Inflamed Lacrimal Glands Is Mediated by Vascular Cell Adhesion Molecule-1/{alpha}4{beta}1 Integrin, Peripheral Node Addressin/L-Selectin, and Lymphocyte Function-Associated Antigen-1 Adhesion Pathways. Am. J. Pathol. 159: 671-681 [Abstract] [Full Text]  
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• Baekkevold, E. S., Yamanaka, T., Palframan, R. T., Carlsen, H. S., Reinholt, F. P., von Andrian, U. H., Brandtzaeg, P., Haraldsen, G. (2001). The CCR7 Ligand ELC (CCL19) Is Transcytosed in High Endothelial Venules and Mediates T Cell Recruitment. JEM 193: 1105-1112 [Abstract] [Full Text]  
• Lee, C.-k., Kim, K., Welniak, L. A., Murphy, W. J., Muegge, K., Durum, S. K. (2001). Thymic emigrants isolated by a new method possess unique phenotypic and functional properties. Blood 97: 1360-1369 [Abstract] [Full Text]  
• Campbell, J. J., Murphy, K. E., Kunkel, E. J., Brightling, C. E., Soler, D., Shen, Z., Boisvert, J., Greenberg, H. B., Vierra, M. A., Goodman, S. B., Genovese, M. C., Wardlaw, A. J., Butcher, E. C., Wu, L. (2001). CCR7 Expression and Memory T Cell Diversity in Humans. J. Immunol. 166: 877-884 [Abstract] [Full Text]  
• von Andrian, U. H., Mackay, C. R. (2000). T-Cell Function and Migration -- Two Sides of the Same Coin. NEJM 343: 1020-1034 [Full Text]  
• Dixon, A. E., Mandac, J. B., Martin, P. J., Hackman, R. C., Madtes, D. K., Clark, J. G. (2000). Adherence of adoptively transferred alloreactive Th1 cells in lung: partial dependence on LFA-1 and ICAM-1. Am. J. Physiol. Lung Cell. Mol. Physiol. 279: L583-L591 [Abstract] [Full Text]  
• Grabovsky, V., Feigelson, S., Chen, C., Bleijs, D. A., Peled, A., Cinamon, G., Baleux, F., Arenzana-Seisdedos, F., Lapidot, T., van Kooyk, Y., Lobb, R. R., Alon, R. (2000). Subsecond Induction of {alpha}4 Integrin Clustering by Immobilized Chemokines Stimulates Leukocyte Tethering and Rolling on Endothelial Vascular Cell Adhesion Molecule 1 under Flow Conditions. JEM 192: 495-506 [Abstract] [Full Text]  
• Drillenburg, P., Pals, S. T. (2000). Cell adhesion receptors in lymphoma dissemination. Blood 95: 1900-1910 [Abstract] [Full Text]  
• Faveeuw, C., Di Mauro, M. E., Price, A. A., Ager, A. (2000). Roles of {alpha}4 integrins/VCAM-1 and LFA-1/ICAM-1 in the binding and transendothelial migration of T lymphocytes and T lymphoblasts across high endothelial venules. Int Immunol 12: 241-251 [Abstract] [Full Text]  
• Stein, J. V., Rot, A., Luo, Y., Narasimhaswamy, M., Nakano, H., Gunn, M. D., Matsuzawa, A., Quackenbush, E. J., Dorf, M. E., von Andrian, U. H. (2000). The CC Chemokine Thymus-derived Chemotactic Agent 4 (TCA-4, Secondary Lymphoid Tissue Chemokine, 6Ckine, Exodus-2) Triggers Lymphocyte Function-associated Antigen 1-mediated Arrest of Rolling T Lymphocytes in Peripheral Lymph Node High Endothelial Venules. JEM 191: 61-76 [Abstract] [Full Text]  
• Warnock, R.A., Campbell, J.J., Dorf, M.E., Matsuzawa, A., McEvoy, L.M., Butcher, E.C. (2000). The Role of Chemokines in the Microenvironmental Control of T versus B Cell Arrest in Peyer's Patch High Endothelial Venules. JEM 191: 77-88 [Abstract] [Full Text]  
• Hasegawa, H., Nomura, T., Kohno, M., Tateishi, N., Suzuki, Y., Maeda, N., Fujisawa, R., Yoshie, O., Fujita, S. (2000). Increased chemokine receptor CCR7/EBI1 expression enhances the infiltration of lymphoid organs by adult T-cell leukemia cells. Blood 95: 30-38 [Abstract] [Full Text]  
• Petersen, F., Bock, L., Flad, H.-D., Brandt, E. (1999). Platelet Factor 4-Induced Neutrophil-Endothelial Cell Interaction: Involvement of Mechanisms and Functional Consequences Different From Those Elicited by Interleukin-8. Blood 94: 4020-4028 [Abstract] [Full Text]  
• Robert, C., Kupper, T. S. (1999). Inflammatory Skin Diseases, T Cells, and Immune Surveillance. NEJM 341: 1817-1828 [Full Text]  
• Izawa, D., Tanaka, T., Saito, K., Ogihara, H., Usui, T., Kawamoto, S., Matsubara, K., Okubo, K., Miyasaka, M. (1999). Expression profile of active genes in mouse lymph node high endothelial cells. Int Immunol 11: 1989-1998 [Abstract] [Full Text]  
• Johnson, G. G., Mikulowska, A., Butcher, E. C., McEvoy, L. M., Michie, S. A. (1999). Anti-CD43 Monoclonal Antibody L11 Blocks Migration of T Cells to Inflamed Pancreatic Islets and Prevents Development of Diabetes in Nonobese Diabetic Mice. J. Immunol. 163: 5678-5685 [Abstract] [Full Text]  
• Rosen, S. D. (1999). Endothelial Ligands for L-Selectin : From Lymphocyte Recirculation to Allograft Rejection. Am. J. Pathol. 155: 1013-1020 [Full Text]  

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