The Journal of Experimental Medicine
VeriKine-HS Human IFN-Beta
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 226K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Griffin, T. A.
Right arrow Articles by Colbert, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Griffin, T. A.
Right arrow Articles by Colbert, R. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/1998/1/97/ $5.00
The Journal of Experimental Medicine, Volume 187, Number 1, January 5, 1998 97-104

Article

Immunoproteasome Assembly: Cooperative Incorporation of Interferon {gamma} (IFN-{gamma})–inducible Subunits

Thomas A. Griffin*, Dipankar Nandi{ddagger}, Miguel Cruz{ddagger}, Hans Jörg Fehling||, Luc Van Kaer, John J. Monaco{ddagger},§, and Robert A. Colbert*

From the * William S. Rowe Division of Rheumatology, Children's Hospital Medical Center, Cincinnati, Ohio 45229; {ddagger} Department of Molecular Genetics and the § Howard Hughes Medical Institute, University of Cincinnati School of Medicine, Cincinnati, Ohio 45267; || Basel Institute for Immunology, CH-4005 Basel, Switzerland; and the Howard Hughes Medical Institute and Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

LMP2, LMP7, and MECL are interferon {gamma}–inducible catalytic subunits of vertebrate 20S proteasomes, which can replace constitutive catalytic subunits (delta, X, and Z, respectively) during proteasome biogenesis. We demonstrate that MECL requires LMP2 for efficient incorporation into preproteasomes, and preproteasomes containing LMP2 and MECL require LMP7 for efficient maturation. The latter effect depends on the presequence of LMP7, but not on LMP7 catalytic activity. This cooperative mechanism favors the assembly of homogeneous "immunoproteasomes" containing all three inducible subunits, suggesting that these subunits act in concert to enhance proteasomal generation of major histocompatibility complex class I–binding peptides.

Address correspondence to Dr. Robert A. Colbert, William S. Rowe Division of Rheumatology, Children's Hospital Medical Center, 3333 Burnet Ave., Cincinnati, OH 45229. Phone: 513-636-4934; Fax: 513-636-5831; E-mail: bob.colbert{at}chmcc.org

1 Abbreviation used in this paper: NEPHGE, nonequilibrium pH gradient electrophoresis.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS