The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1997/11/1481/ $5.00
The Journal of Experimental Medicine, Volume 186, Number 9, November 3, 1997 1481-1486

Article

Antigen Presentation by Dendritic Cells after Immunization with DNA Encoding a Major Histocompatibility Complex Class II–restricted Viral Epitope

Sofia Casares*, Kayo Inaba{ddagger}, Teodor-Doru Brumeanu*, Ralph M. Steinman§, and Constantin A. Bona*

From the * Department of Microbiology, Mount Sinai School of Medicine, New York 10029; the {ddagger} Laboratory of Immunology, Department of Zoology, Faculty of Science, Kyoto University, Japan; and the § Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York 10021

Intramuscular and intracutaneous immunization with naked DNA can vaccinate animals to the encoded proteins, but the underlying mechanisms of antigen presentation are unclear. We used DNA that encodes an A/PR/8/34 influenza peptide for CD4 T cells and that elicits protective antiviral immunity. DNA-transfected, cultured muscle cells released the influenza polypeptide, which then could be presented on the major histocompatibility complex class II molecules of dendritic cells. When DNA was injected into muscles or skin, and antigen-presenting cells were isolated from either the draining lymph nodes or the skin, dendritic, but not B, cells presented antigen to T cells and carried plasmid DNA. We suggest that the uptake of DNA and/or the protein expressed by dendritic cells triggers immune responses to DNA vaccines.

Address correspondence to C.A. Bona, Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, Box 1124, New York, NY 10029. Phone: 212-241-6924; FAX: 212-423-0711; E-mail: bona@.msvaz.mssm.edu

1 Abbreviations used in this paper: HA, hemagglutinin; pC, plasmid control; TcH, T cell hybridoma.


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