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From the * Division of Tumor Virology, and the Previous studies have shown that integrin Division of Molecular and Cellular Biology,
Dana-Farber Cancer Institute, Boston, Massachusetts 02115; and the § Department of Cell Biology,
Duke University Medical Center, Durham, North Carolina 27710
chain tails make strong positive contributions to
integrin-mediated cell adhesion. We now show here that integrin 4 tail deletion markedly impairs static cell adhesion by a mechanism that does not involve altered binding of soluble vascular cell adhesion molecule 1 ligand. Instead, truncation of the 4 cytoplasmic domain caused a
severe deficiency in integrin accumulation into cell surface clusters, as induced by ligand and/
or antibodies. Furthermore, 4 tail deletion also significantly decreased the membrane diffusivity of 4
1, as determined by a single particle tracking technique. Notably, low doses of cytochalasin D partially restored the deficiency in cell adhesion seen upon 4 tail deletion. Together, these results suggest that 4 tail deletion exposes the 1 cytoplasmic domain, leading to
cytoskeletal associations that apparently restrict integrin lateral diffusion and accumulation into
clusters, thus causing reduced static cell adhesion. Our demonstration of integrin adhesive activity regulated through receptor diffusion/clustering (rather than through altered ligand binding affinity) may be highly relevant towards the understanding of inside-out signaling mechanisms for 1 integrins.
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