The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1997/10/1201/ $5.00
The Journal of Experimental Medicine, Volume 186, Number 8, October 20, 1997 1201-1212

Articles

Upregulation of Interleukin 8 by Oxygen-deprived Cells in Glioblastoma Suggests a Role in Leukocyte Activation, Chemotaxis, and Angiogenesis

Isabelle Desbaillets, Annie-Claire Diserens, Nicolas de Tribolet, Marie-France Hamou, and Erwin G. Van Meir

From the Laboratory of Tumor Biology and Genetics, Neurosurgery Department, University Hospital (CHUV), 1011 Lausanne, Switzerland

Leukocyte infiltration and necrosis are two biological phenomena associated with the development of neovascularization during the malignant progression of human astrocytoma. Here, we demonstrate expression of interleukin (IL)-8, a cytokine with chemotactic and angiogenic properties, and of IL-8–binding receptors in astrocytoma. IL-8 expression is first observed in low grade astrocytoma in perivascular tumor areas expressing inflammatory cytokines. In glioblastoma, it further localizes to oxygen-deprived cells surrounding necrosis. Hypoxic/anoxic insults on glioblastoma cells in vitro using anaerobic chamber systems or within spheroids developing central necrosis induced an increase in IL-8 messenger RNA (mRNA) and protein expression. mRNA for IL-8–binding chemokine receptors CXCR1, CXCR2, and the Duffy antigen receptor for chemokines (DARC) were found in all astrocytoma grades by reverse transcription/PCR analysis. In situ hybridization and immunohistochemistry localized DARC expression on normal brain and tumor microvascular cells and CXCR1 and CXCR2 expression to infiltrating leukocytes. These results support a model where IL-8 expression is initiated early in astrocytoma development through induction by inflammatory stimuli and later in tumor progression increases due to reduced microenvironmental oxygen pressure. Augmented IL-8 would directly and/or indirectly promote angiogenesis by binding to DARC and by inducing leukocyte infiltration and activation by binding to CXCR1 and CXCR2.

Address correspondence to Erwin G. Van Meir, Laboratory of Tumor Biology and Genetics, Neurosurgery Department, University Hospital (CHUV), 1011 Lausanne, Switzerland. Phone: +41-21-314-2582; FAX: +41-21-314-2587; E-mail: evanmeir{at}hola.hospvd.ch

1 Abbreviations used in this paper: DARC, Duffy antigen receptor for chemokines; GFAP, glial fibrillary acidic protein; mRNA, messenger RNA; RT, reverse transcription; VEGF, vascular endothelial growth factor.


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