The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1997/10/1177/ $5.00
The Journal of Experimental Medicine, Volume 186, Number 7, October 6, 1997 1177-1182

Brief Definitive Reports

Bone Marrow–generated Dendritic Cells Pulsed with Tumor Extracts or Tumor RNA Induce Antitumor Immunity against Central Nervous System Tumors

David M. Ashley*, Brenda Faiola||,§, Smita Nair||, Laura P. Hale{ddagger}, Darell D. Bigner{ddagger}, and Eli Gilboa||,§

From the * Department of Pediatrics, {ddagger} Department of Pathology, § Department of Immunology, and || Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710

Recent studies have shown that the brain is not a barrier to successful active immunotherapy that uses gene-modified autologous tumor cell vaccines. In this study, we compared the efficacy of two types of vaccines for the treatment of tumors within the central nervous system (CNS): dendritic cell (DC)-based vaccines pulsed with either tumor extract or tumor RNA, and cytokine gene–modified tumor vaccines. Using the B16/F10 murine melanoma (B16) as a model for CNS tumor, we show that vaccination with bone marrow–generated DCs, pulsed with either B16 cell extract or B16 total RNA, can induce specific cytotoxic T lymphocytes against B16 tumor cells. Both types of DC vaccines were able to protect animals from tumors located in the CNS. DC-based vaccines also led to prolonged survival in mice with tumors placed before the initiation of vaccine therapy. The DC-based vaccines were at least as effective, if not more so, as vaccines containing B16 tumor cells in which the granulocytic macrophage colony-stimulating factor gene had been modified. These data support the use of DC-based vaccines for the treatment of patients with CNS tumors.

Address correspondence to Dr. Eli Gilboa, Duke University Medical Center, Department of Surgery, Box 2601, Medical Sciences Research Bldg. (MSRB), Rm 401, Durham, NC, 27710. Phone: 919-684-6465; FAX: 919-681-7970.

The authors acknowledge the expert technical assistance of both Paula Greer and Jie Li. Ann Tamariz provided editorial assistance.


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