Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 101K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tanchot, C. Articles by Rocha, B. Search for Related Content PubMed PubMed Citation Articles by Tanchot, C. Articles by Rocha, B. Social Bookmarking                            What's this?

Articles

From the U345 Institut National de la Sante et de la Recherche Medicale, Institut Necker, 156, 75730 Paris Cedex 15, France

We investigated the role of continuous thymus output in the shaping of mature T cell repertoires by studying in vivo the survival of a single clone of mature Rag2-deficient T cell receptor (TCR) transgenic cells at different stages of activation in the absence or presence of thymus export. In the absence of thymus export, TCR-transgenic lymphocytes survived indefinitely in the peripheral pools. When new lymphocytes were produced in the thymus and migrated to the periphery, resident memory T cells were maintained in constant numbers, whereas naive and self-reactive T cells were replaced by recent thymus migrants.

This T cell renewal ensured both the efficiency of recall responses to antigens as memory T cells persisted independently of thymus output, and the capacity of the immune system to respond to new antigen stimulation as the naive T cell pool was continuously renewed. Our results also indicate that thymus export is required to control the number of self-reactive peripheral T cells that may invade the peripheral pools if thymus output fails.

¹ Abbreviations used in this paper: BM, bone marrow; DP, double positive; mRNA, messenger RNA; R, rads; SP, single positive; Tg, transgenic.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Gourdain, P., Gregoire, S., Iken, S., Bachy, V., Dorban, G., Chaigneau, T., Debiec, H., Bergot, A.-S., Renault, I., Aucouturier, P., Carnaud, C. (2009). Adoptive Transfer of T Lymphocytes Sensitized against the Prion Protein Attenuates Prion Invasion in Scrapie-Infected Mice. J. Immunol. 183: 6619-6628 [Abstract] [Full Text]  
• Leitao, C., Freitas, A. A., Garcia, S. (2009). The Role of TCR Specificity and Clonal Competition During Reconstruction of the Peripheral T Cell Pool. J. Immunol. 182: 5232-5239 [Abstract] [Full Text]  
• Ahmed, M., Lanzer, K. G., Yager, E. J., Adams, P. S., Johnson, L. L., Blackman, M. A. (2009). Clonal Expansions and Loss of Receptor Diversity in the Naive CD8 T Cell Repertoire of Aged Mice. J. Immunol. 182: 784-792 [Abstract] [Full Text]  
• Bourgeois, C., Hao, Z., Rajewsky, K., Potocnik, A. J., Stockinger, B. (2008). Ablation of thymic export causes accelerated decay of naive CD4 T cells in the periphery because of activation by environmental antigen. Proc. Natl. Acad. Sci. USA 105: 8691-8696 [Abstract] [Full Text]  
• Metzler, B., Gfeller, P., Wieczorek, G., Li, J., Nuesslein-Hildesheim, B., Katopodis, A., Mueller, M., Brinkmann, V. (2008). Modulation of T cell homeostasis and alloreactivity under continuous FTY720 exposure. Int Immunol 20: 633-644 [Abstract] [Full Text]  
• Ryan, C. M., Schell, T. D. (2006). Accumulation of CD8+ T Cells in Advanced-Stage Tumors and Delay of Disease Progression following Secondary Immunization against an Immunorecessive Epitope. J. Immunol. 177: 255-267 [Abstract] [Full Text]  
• Martin, B., Becourt, C., Bienvenu, B., Lucas, B. (2006). Self-recognition is crucial for maintaining the peripheral CD4+ T-cell pool in a nonlymphopenic environment. Blood 108: 270-277 [Abstract] [Full Text]  
• Berzins, S. P., McNab, F. W., Jones, C. M., Smyth, M. J., Godfrey, D. I. (2006). Long-Term Retention of Mature NK1.1+ NKT Cells in the Thymus. J. Immunol. 176: 4059-4065 [Abstract] [Full Text]  
• Dowling, M. R, Milutinovic, D., Hodgkin, P. D (2005). Modelling cell lifespan and proliferation: is likelihood to die or to divide independent of age?. J R Soc Interface 2: 517-526 [Abstract] [Full Text]  
• Huang, Y., Obholzer, N., Fayad, R., Qiao, L. (2005). Turning On/Off Tumor-Specific CTL Response during Progressive Tumor Growth. J. Immunol. 175: 3110-3116 [Abstract] [Full Text]  
• Kayser, C, Alberto, F L, da Silva, N P, Andrade, L E. (2004). Decreased number of T cells bearing TCR rearrangement excision circles (TREC) in active recent onset systemic lupus erythematosus. Lupus 13: 906-911 [Abstract]  
• Fridkis-Hareli, M., Reche, P. A., Reinherz, E. L. (2004). Peptide Variants of Viral CTL Epitopes Mediate Positive Selection and Emigration of Ag-Specific Thymocytes In Vivo. J. Immunol. 173: 1140-1150 [Abstract] [Full Text]  
• Zippelius, A., Batard, P., Rubio-Godoy, V., Bioley, G., Lienard, D., Lejeune, F., Rimoldi, D., Guillaume, P., Meidenbauer, N., Mackensen, A., Rufer, N., Lubenow, N., Speiser, D., Cerottini, J.-C., Romero, P., Pittet, M. J. (2004). Effector Function of Human Tumor-Specific CD8 T Cells in Melanoma Lesions: A State of Local Functional Tolerance. Cancer Res. 64: 2865-2873 [Abstract] [Full Text]  
• Blais, M.-E., Gerard, G., Martinic, M. M., Roy-Proulx, G., Zinkernagel, R. M., Perreault, C. (2004). Do thymically and strictly extrathymically developing T cells generate similar immune responses?. Blood 103: 3102-3110 [Abstract] [Full Text]  
• Vivinus-Nebot, M., Rousselle, P., Breittmayer, J.-P., Cenciarini, C., Berrih-Aknin, S., Spong, S., Nokelainen, P., Cottrez, F., Marinkovich, M. P., Bernard, A. (2004). Mature Human Thymocytes Migrate on Laminin-5 with Activation of Metalloproteinase-14 and Cleavage of CD44. J. Immunol. 172: 1397-1406 [Abstract] [Full Text]  
• Gebuhr, T. C., Kovalev, G. I., Bultman, S., Godfrey, V., Su, L., Magnuson, T. (2003). The Role of Brg1, a Catalytic Subunit of Mammalian Chromatin-remodeling Complexes, in T Cell Development. JEM 198: 1937-1949 [Abstract] [Full Text]  
• Permar, S. R., Moss, W. J., Ryon, J. J., Douek, D. C., Monze, M., Griffin, D. E. (2003). Increased Thymic Output during Acute Measles Virus Infection. J. Virol. 77: 7872-7879 [Abstract] [Full Text]  
• Williams, M. A., Adams, A. B., Walsh, M. B., Shirasugi, N., Onami, T. M., Pearson, T. C., Ahmed, R., Larsen, C. P. (2003). Primary and Secondary Immunocompetence in Mixed Allogeneic Chimeras. J. Immunol. 170: 2382-2389 [Abstract] [Full Text]  
• Huang, C.-C., Shah, S., Nguyen, P., Altman, J. D., Blackman, M. A. (2002). Bacterial Superantigen Exposure after Resolution of Influenza Virus Infection Perturbs the Virus-Specific Memory CD8+-T-Cell Repertoire. J. Virol. 76: 6852-6856 [Abstract] [Full Text]  
• Tanchot, C., Le Campion, A., Martin, B., Leaument, S., Dautigny, N., Lucas, B. (2002). Conversion of Naive T Cells to a Memory-Like Phenotype in Lymphopenic Hosts Is Not Related to a Homeostatic Mechanism That Fills the Peripheral Naive T Cell Pool. J. Immunol. 168: 5042-5046 [Abstract] [Full Text]  
• Le Campion, A., Bourgeois, C., Lambolez, F., Martin, B., Leaument, S., Dautigny, N., Tanchot, C., Penit, C., Lucas, B. (2002). Naive T cells proliferate strongly in neonatal mice in response to self-peptide/self-MHC complexes. Proc. Natl. Acad. Sci. USA 99: 4538-4543 [Abstract] [Full Text]  
• Ge, Q., Hu, H., Eisen, H. N., Chen, J. (2002). Different contributions of thymopoiesis and homeostasis-driven proliferation to the reconstitution of naive and memory T cell compartments. Proc. Natl. Acad. Sci. USA 99: 2989-2994 [Abstract] [Full Text]  
• Mora, A. L., Stanley, S., Armistead, W., Chan, A. C., Boothby, M. (2001). Inefficient ZAP-70 Phosphorylation and Decreased Thymic Selection In Vivo Result from Inhibition of NF-{kappa}B/Rel. J. Immunol. 167: 5628-5635 [Abstract] [Full Text]  
• Cunningham, C. P., Kimpton, W. G., Fernando, A., Cahill, R. N. P. (2001). Neonatal thymectomy identifies two major pools of sessile and recirculating peripheral T cells which appear to be under separate homeostatic control. Int Immunol 13: 1351-1359 [Abstract] [Full Text]  
• Gudmundsdottir, H., Turka, L. A. (2001). A Closer Look at Homeostatic Proliferation of CD4+ T Cells: Costimulatory Requirements and Role in Memory Formation. J. Immunol. 167: 3699-3707 [Abstract] [Full Text]  
• Gorochov, G., Neumann, A. U., Parizot, C., Li, T., Katlama, C., Debre, P. (2001). Down-regulation of CD8+ T-cell expansions in patients with human immunodeficiency virus infection receiving highly active combination therapy. Blood 97: 1787-1795 [Abstract] [Full Text]  
• Mackall, C. L., Fry, T. J., Bare, C., Morgan, P., Galbraith, A., Gress, R. E. (2001). IL-7 increases both thymic-dependent and thymic-independent T-cell regeneration after bone marrow transplantation. Blood 97: 1491-1497 [Abstract] [Full Text]  
• Foss, D. L., Donskoy, E., Goldschneider, I. (2001). The Importation of Hematogenous Precursors by the Thymus Is a Gated Phenomenon in Normal Adult Mice. JEM 193: 365-374 [Abstract] [Full Text]  
• Seabrook, T. J., Hein, W. R., Dudler, L., Young, A. J. (2000). Splenectomy selectively affects the distribution and mobility of the recirculating lymphocyte pool. Blood 96: 1180-1183 [Abstract] [Full Text]  
• Boileau, C., Houde, M., Dulude, G., Clegg, C. H., Perreault, C. (2000). Regulation of Extrathymic T Cell Development and Turnover by Oncostatin M. J. Immunol. 164: 5713-5720 [Abstract] [Full Text]  
• Annacker, O., Burlen-Defranoux, O., Pimenta-Araujo, R., Cumano, A., Bandeira, A. (2000). Regulatory CD4 T Cells Control the Size of the Peripheral Activated/Memory CD4 T Cell Compartment. J. Immunol. 164: 3573-3580 [Abstract] [Full Text]  
• Muranski, P., Chmielowski, B., Ignatowicz, L. (2000). Mature CD4+ T Cells Perceive a Positively Selecting Class II MHC/Peptide Complex in the Periphery. J. Immunol. 164: 3087-3094 [Abstract] [Full Text]  
• Zujewski, J., Horak, I. D., Bol, C. J., Woestenborghs, R., Bowden, C., End, D. W., Piotrovsky, V. K., Chiao, J., Belly, R. T., Todd, A., Kopp, W. C., Kohler, D. R., Chow, C., Noone, M., Hakim, F. T., Larkin, G., Gress, R. E., Nussenblatt, R. B., Kremer, A. B., Cowan, K. H. (2000). Phase I and Pharmacokinetic Study of Farnesyl Protein Transferase Inhibitor R115777 in Advanced Cancer. JCO 18: 927-927 [Abstract] [Full Text]  
• Chmielowski, B., Muranski, P., Kisielow, P., Ignatowicz, L. (2000). On the role of high- and low-abundance class II MHC-peptide complexes in the thymic positive selection of CD4+ T cells. Int Immunol 12: 67-72 [Abstract] [Full Text]  
• Kieper, W. C., Jameson, S. C. (1999). Homeostatic expansion and phenotypic conversion of naive T cells in response to self peptide/MHC ligands. Proc. Natl. Acad. Sci. USA 96: 13306-13311 [Abstract] [Full Text]  
• Spaner, D., Sheng-Tanner, X., Raju, K., Rabinovich, B., Messner, H., Miller, R. G. (1999). Long-term persistence of IL-2-unresponsive allogeneic T cells in sublethally irradiated SCID mice. Int Immunol 11: 1601-1614 [Abstract] [Full Text]  
• Borghans, J. A. M., Taams, L. S., Wauben, M. H. M., de Boer, R. J. (1999). Competition for antigenic sites during T cell proliferation: A mathematical interpretation of in vitro data. Proc. Natl. Acad. Sci. USA 96: 10782-10787 [Abstract] [Full Text]  
• Berzins, S. P., Godfrey, D. I., Miller, J. F. A. P., Boyd, R. L. (1999). A central role for thymic emigrants in peripheral T cell homeostasis. Proc. Natl. Acad. Sci. USA 96: 9787-9791 [Abstract] [Full Text]  
• Di Rosa, F., Ramaswamy, S., Ridge, J. P., Matzinger, P. (1999). On the Lifespan of Virgin T Lymphocytes. J. Immunol. 163: 1253-1257 [Abstract] [Full Text]  
• Brochu, S., Rioux-Masse, B., Roy, J., Roy, D.-C., Perreault, C. (1999). Massive Activation-Induced Cell Death of Alloreactive T Cells With Apoptosis of Bystander Postthymic T Cells Prevents Immune Reconstitution in Mice With Graft-Versus-Host Disease. Blood 94: 390-400 [Abstract] [Full Text]  
• Blish, C. A., Dillon, S. R., Farr, A. G., Fink, P. J. (1999). Anergic CD8+ T Cells Can Persist and Function In Vivo. J. Immunol. 163: 155-164 [Abstract] [Full Text]  
• Chmielowski, B., Muranski, P., Ignatowicz, L. (1999). In the Normal Repertoire of CD4+ T Cells, a Single Class II MHC/Peptide Complex Positively Selects TCRs with Various Antigen Specificities. J. Immunol. 162: 95-105 [Abstract] [Full Text]  
• Antia, R., Pilyugin, S. S., Ahmed, R. (1998). Models of immune memory: On the role of cross-reactive stimulation, competition, and homeostasis in maintaining immune memory. Proc. Natl. Acad. Sci. USA 95: 14926-14931 [Abstract] [Full Text]  
• Berzins, S.P., Boyd, R.L., Miller, J.F.A.P. (1998). The Role of the Thymus and Recent Thymic Migrants in the Maintenance of the Adult Peripheral Lymphocyte Pool. JEM 187: 1839-1848 [Abstract] [Full Text]  
• Le Campion, A., Bourgeois, C., Lambolez, F., Martin, B., Leaument, S., Dautigny, N., Tanchot, C., Penit, C., Lucas, B. (2002). Naive T cells proliferate strongly in neonatal mice in response to self-peptide/self-MHC complexes. Proc. Natl. Acad. Sci. USA 99: 4538-4543 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS