The Journal of Experimental Medicine
Northwestern University Inflammation Research
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© The Rockefeller University Press, 0022-1007/1997/9/909/ $5.00
The Journal of Experimental Medicine, Volume 186, Number 6, September 15, 1997 909-920

Articles

Eosinophil Lipid Bodies: Specific, Inducible Intracellular Sites for Enhanced Eicosanoid Formation

Patricia T. Bozza*,{ddagger}, Wengui Yu*,{ddagger}, John F. Penrose||, Ellen S. Morgan*,§, Ann M. Dvorak*,§, and Peter F. Weller*,{ddagger}

From the * Harvard Thorndike Laboratory and Charles A. Dana Research Institute, {ddagger} Department of Medicine and § Department of Pathology, Beth Israel Deaconess Medical Center, and || Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02215

The specific intracellular sites at which enzymes act to generate arachidonate-derived eicosanoid mediators of inflammation are uncertain. We evaluated the formation and function of cytoplasmic lipid bodies. Lipid body formation in eosinophils was a rapidly (<1 h) inducible response which was platelet-activating factor (PAF) receptor–mediated, involved signaling through protein kinase C, and required new protein synthesis. In intact and enucleated eosinophils, the PAF-induced increases in lipid body numbers correlated with enhanced production of both lipoxygenase- and cyclooxygenase-derived eicosanoids. All principal eosinophil eicosanoid-forming enzymes, 5-lipoxygenase, leukotriene C4 synthase, and cyclooxygenase, were immunolocalized to native as well as newly induced lipid bodies in intact and enucleated eosinophils. Thus, lipid bodies are structurally distinct, inducible, nonnuclear sites for enhanced synthesis of paracrine eicosanoid mediators of inflammation.

Address correspondence to Dr. Peter F. Weller, Beth Israel Deaconess Medical Center, 330 Brookline Ave., DA-617, Boston, MA 02215. Phone: 617-667-3307; FAX: 617-277-6061; E-mail: pweller{at}bidmc.harvard.edu

1 Abbreviations used in this paper: cPLA2, cytosolic phospholipase A2; COX, cyclooxygenase; HES, hypereosinophilic syndrome; LT, leukotriene; LO, lipoxygenase; PAF, platelet-activating factor; PKC, protein kinase C; TBS-BSA, Tris-buffered saline containing 0.1% BSA.


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