© The Rockefeller University Press, 0022-1007/1997/8/731/ $5.00
The Journal of Experimental Medicine, Volume 186, Number 5, August 29, 1997 731-737
Epstein-Barr Virus Binding to CD21 Activates the Initial Viral Promoter via NF-
B Induction
Naoyuki Sugano,
Weiping Chen,
M. Luisa Roberts, and
Neil R. Cooper
From the Scripps Research Institute, Department of Immunology, La Jolla, California 92037
Epstein-Barr virus (EBV), an oncogenic human herpesvirus, binds to and infects normal human B lymphocytes via CD21, the CR2 complement receptor. Studies of the mechanisms that enable EBV to infect nonactivated, noncycling B cells provide compelling evidence for a sequence of events in which EBV binding to CD21 on purified resting human B cells rapidly activates the NF-
B transcription factor, which, in turn, binds to and mediates transcriptional activation of Wp, the initial viral latent gene promoter. Thus, EBV binding to its cellular receptor on resting B cells triggers an NF-
B–dependent intracellular signaling pathway which is required for infection.
Address correspondence to Dr. Neil R. Cooper, Department of Immunology, IMM-19, 10550 North Torrey Pines Rd., La Jolla, CA 92037. Phone: 619-784-8152; FAX: 619-784-8472; E-mail: nrcooper @scripps.edu. The current address of N. Sugano is Nihon University School of Dentistry, Department of Periodontology, 1-8-13 Kanda-Surugadai Chiyoda-ku, Tokyo 101, Japan. The current address of M.L. Roberts is Isis Pharmaceuticals, 2282 Faraday Ave., Carlsbad, CA 92008.
1 Abbreviations used in this paper: CAT, chloramphenicol acetyltransferase; EBNA, Epstein-Barr virus nuclear antigen; EMSA, electrophoretic mobility shift assay; LTR, long-terminal repeat; PKC, protein kinase C; rpL32, ribosomal protein L32; RT, reverse transcriptase.

CiteULike
Complore
Connotea
Del.icio.us
Digg
Facebook
Reddit
Technorati
Twitter What's this?
This article has been cited by other articles:
-
Johansson, P., Jansson, A., Ruetschi, U., Rymo, L.
(2009). Nuclear Factor-{kappa}B Binds to the Epstein-Barr Virus LMP1 Promoter and Upregulates Its Expression. J. Virol.
83: 1393-1401
[Abstract]
[Full Text]
-
Martin, H. J., Lee, J. M., Walls, D., Hayward, S. D.
(2007). Manipulation of the Toll-Like Receptor 7 Signaling Pathway by Epstein-Barr Virus. J. Virol.
81: 9748-9758
[Abstract]
[Full Text]
-
Tierney, R., Nagra, J., Hutchings, I., Shannon-Lowe, C., Altmann, M., Hammerschmidt, W., Rickinson, A., Bell, A.
(2007). Epstein-Barr Virus Exploits BSAP/Pax5 To Achieve the B-Cell Specificity of Its Growth-Transforming Program. J. Virol.
81: 10092-10100
[Abstract]
[Full Text]
-
Hutt-Fletcher, L. M.
(2007). Epstein-Barr Virus Entry. J. Virol.
81: 7825-7832
[Full Text]
-
Gaudreault, E., Fiola, S., Olivier, M., Gosselin, J.
(2007). Epstein-Barr Virus Induces MCP-1 Secretion by Human Monocytes via TLR2. J. Virol.
81: 8016-8024
[Abstract]
[Full Text]
-
Montag, C., Wagner, J., Gruska, I., Hagemeier, C.
(2006). Human Cytomegalovirus Blocks Tumor Necrosis Factor Alpha- and Interleukin-1{beta}-Mediated NF-{kappa}B Signaling. J. Virol.
80: 11686-11698
[Abstract]
[Full Text]
-
Hsiao, F. C., Lin, M., Tai, A., Chen, G., Huber, B. T.
(2006). Cutting Edge: Epstein-Barr Virus Transactivates the HERV-K18 Superantigen by Docking to the Human Complement Receptor 2 (CD21) on Primary B Cells. J. Immunol.
177: 2056-2060
[Abstract]
[Full Text]
-
Asokan, R., Hua, J., Young, K. A., Gould, H. J., Hannan, J. P., Kraus, D. M., Szakonyi, G., Grundy, G. J., Chen, X. S., Crow, M. K., Holers, V. M.
(2006). Characterization of Human Complement Receptor Type 2 (CR2/CD21) as a Receptor for IFN-{alpha}: A Potential Role in Systemic Lupus Erythematosus. J. Immunol.
177: 383-394
[Abstract]
[Full Text]
-
Shannon-Lowe, C. D., Neuhierl, B., Baldwin, G., Rickinson, A. B., Delecluse, H.-J.
(2006). From the Cover: Resting B cells as a transfer vehicle for Epstein-Barr virus infection of epithelial cells. Proc. Natl. Acad. Sci. USA
103: 7065-7070
[Abstract]
[Full Text]
-
Shannon-Lowe, C., Baldwin, G., Feederle, R., Bell, A., Rickinson, A., Delecluse, H.-J.
(2005). Epstein-Barr virus-induced B-cell transformation: quantitating events from virus binding to cell outgrowth. J. Gen. Virol.
86: 3009-3019
[Abstract]
[Full Text]
-
Chang, E. T., Zheng, T., Weir, E. G., Borowitz, M., Mann, R. B., Spiegelman, D., Mueller, N. E.
(2004). Aspirin and the Risk of Hodgkin's Lymphoma in a Population-Based Case-Control Study. JNCI J Natl Cancer Inst
96: 305-315
[Abstract]
[Full Text]
-
Melchjorsen, J., Sorensen, L. N., Paludan, S. R.
(2003). Expression and function of chemokines during viral infections: from molecular mechanisms to in vivo function. J. Leukoc. Biol.
74: 331-343
[Abstract]
[Full Text]
-
Mogensen, T. H., Melchjorsen, J., Hollsberg, P., Paludan, S. R.
(2003). Activation of NF-{kappa}B in Virus-Infected Macrophages Is Dependent on Mitochondrial Oxidative Stress and Intracellular Calcium: Downstream Involvement of the Kinases TGF-{beta}-Activated Kinase 1, Mitogen-Activated Kinase/Extracellular Signal-Regulated Kinase Kinase 1, and I{kappa}B Kinase . J. Immunol.
170: 6224-6233
[Abstract]
[Full Text]
-
Naranatt, P. P., Akula, S. M., Zien, C. A., Krishnan, H. H., Chandran, B.
(2002). Kaposi's Sarcoma-Associated Herpesvirus Induces the Phosphatidylinositol 3-Kinase-PKC-{zeta}-MEK-ERK Signaling Pathway in Target Cells Early during Infection: Implications for Infectivity. J. Virol.
77: 1524-1539
[Abstract]
[Full Text]
-
Tolnay, M., Vereshchagina, L. A., Tsokos, G. C.
(2002). NF-{kappa}B Regulates the Expression of the Human Complement Receptor 2 Gene. J. Immunol.
169: 6236-6243
[Abstract]
[Full Text]
-
Takemoto, M., Shimamoto, T., Isegawa, Y., Yamanishi, K.
(2001). The R3 Region, One of Three Major Repetitive Regions of Human Herpesvirus 6, Is a Strong Enhancer of Immediate-Early Gene U95. J. Virol.
75: 10149-10160
[Abstract]
[Full Text]
-
Payne, E., Bowles, M. R., Don, A., Hancock, J. F., McMillan, N. A. J.
(2001). Human Papillomavirus Type 6b Virus-Like Particles Are Able To Activate the Ras-MAP Kinase Pathway and Induce Cell Proliferation. J. Virol.
75: 4150-4157
[Abstract]
[Full Text]
-
Spender, L. C., Cornish, G. H., Rowland, B., Kempkes, B., Farrell, P. J.
(2001). Direct and Indirect Regulation of Cytokine and Cell Cycle Proteins by EBNA-2 during Epstein-Barr Virus Infection. J. Virol.
75: 3537-3546
[Abstract]
[Full Text]
-
Mogensen, T. H., Paludan, S. R.
(2001). Molecular Pathways in Virus-Induced Cytokine Production. Microbiol. Mol. Biol. Rev.
65: 131-150
[Abstract]
[Full Text]
-
Mossman, K. L., Macgregor, P. F., Rozmus, J. J., Goryachev, A. B., Edwards, A. M., Smiley, J. R.
(2001). Herpes Simplex Virus Triggers and Then Disarms a Host Antiviral Response. J. Virol.
75: 750-758
[Abstract]
[Full Text]
-
Feuillard, J., Schuhmacher, M., Kohanna, S., Asso-Bonnet, M., Ledeur, F., Joubert-Caron, R., Bissieres, P., Polack, A., Bornkamm, G. W., Raphael, M.
(2000). Inducible loss of NF-kappa B activity is associated with apoptosis and Bcl-2 down-regulation in Epstein-Barr virus-transformed B lymphocytes. Blood
95: 2068-2075
[Abstract]
[Full Text]
-
DADDARIO, M., AHMAD, A., XU, J. W., MENEZES, J.
(1999). Epstein-Barr virus envelope glycoprotein gp350 induces NF-{kappa}B activation and IL-1{beta} synthesis in human monocytes-macrophages involving PKC and PI3-K. FASEB J.
13: 2203-2213
[Abstract]
[Full Text]
-
Spender, L. C., Cannell, E. J., Hollyoake, M., Wensing, B., Gawn, J. M., Brimmell, M., Packham, G., Farrell, P. J.
(1999). Control of Cell Cycle Entry and Apoptosis in B Lymphocytes Infected by Epstein-Barr Virus. J. Virol.
73: 4678-4688
[Abstract]
[Full Text]
-
Boyle, K. A., Pietropaolo, R. L., Compton, T.
(1999). Engagement of the Cellular Receptor for Glycoprotein B of Human Cytomegalovirus Activates the Interferon-Responsive Pathway. Mol. Cell. Biol.
19: 3607-3613
[Abstract]
[Full Text]