Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 242K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Knorr, R. Articles by Dustin, M. L. Search for Related Content PubMed PubMed Citation Articles by Knorr, R. Articles by Dustin, M. L. Social Bookmarking                            What's this?

Article

From the Center for Immmunology and Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110

The I domain of lymphocyte function–associated antigen (LFA)-1 contains an intercellular adhesion molecule (ICAM)-1 and ICAM-3 binding site, but the relationship of this site to regulated adhesion is unknown. To study the adhesive properties of the LFA-1 I domain, we stably expressed a GPI-anchored form of this I domain (I-GPI) on the surface of baby hamster kidney cells. I-GPI cells bound soluble ICAM-1 (sICAM-1) with a low avidity and affinity. Flow cell experiments demonstrated a specific rolling interaction of I-GPI cells on bilayers containing purified full length ICAM-1 or ICAM-3. The LFA-1 activating antibody MEM-83, or its Fab fragment, decreased the rolling velocity of I-GPI cells on ICAM-1–containing membranes. In contrast, the interaction of I-GPI cells with ICAM-3 was blocked by MEM-83. Rolling of I-GPI cells was dependent on the presence of Mg²⁺. Mn²⁺ only partially substituted for Mg²⁺, giving rise to a small fraction of rolling cells and increased rolling velocity. This suggests that the I domain acts as a transient, Mg²⁺-dependent binding module that cooperates with another Mn²⁺-stimulated site in LFA-1 to give rise to the stable interaction of intact LFA-1 with ICAM-1.

¹ Abbreviations used in this paper: BHK, baby hamster kidney; I-GPI, GPI anchored form of the LFA-1 I domain; ICAM, intercellular adhesion molecule; LFA, lymphocyte function–associated antigen; PIPLC, phosphatidylinositol-specific phospholipase C.

Portions of this work were previously presented at the meeting of the American Association for Immunologists (AAI) on 3-6 June 1996 in New Orleans, LA.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Zarbock, A., Abram, C. L., Hundt, M., Altman, A., Lowell, C. A., Ley, K. (2008). PSGL-1 engagement by E-selectin signals through Src kinase Fgr and ITAM adapters DAP12 and FcR{gamma} to induce slow leukocyte rolling. JEM 205: 2339-2347 [Abstract] [Full Text]  
• Carreno, R., Li, D., Sen, M., Nira, I., Yamakawa, T., Ma, Q., Legge, G. B. (2008). A Mechanism for Antibody-mediated Outside-in Activation of LFA-1. J. Biol. Chem. 283: 10642-10648 [Abstract] [Full Text]  
• Salas, A., Shimaoka, M., Phan, U., Kim, M., Springer, T. A. (2006). Transition from Rolling to Firm Adhesion Can Be Mimicked by Extension of Integrin {alpha}Lbeta2 in an Intermediate Affinity State. J. Biol. Chem. 281: 10876-10882 [Abstract] [Full Text]  
• Green, C. E., Schaff, U. Y., Sarantos, M. R., Lum, A. F. H., Staunton, D. E., Simon, S. I. (2006). Dynamic shifts in LFA-1 affinity regulate neutrophil rolling, arrest, and transmigration on inflamed endothelium. Blood 107: 2101-2111 [Abstract] [Full Text]  
• Rosenthal-Allieri, M. A., Ticchioni, M., Breittmayer, J. P., Shimizu, Y., Bernard, A. (2005). Influence of {beta}1 Integrin Intracytoplasmic Domains in the Regulation of VLA-4-Mediated Adhesion of Human T Cells to VCAM-1 under Flow Conditions. J. Immunol. 175: 1214-1223 [Abstract] [Full Text]  
• Huang, M., Matthews, K., Siahaan, T. J., Kevil, C. G. (2005). {alpha}L-Integrin I domain cyclic peptide antagonist selectively inhibits T cell adhesion to pancreatic islet microvascular endothelium. Am. J. Physiol. Gastrointest. Liver Physiol. 288: G67-G73 [Abstract] [Full Text]  
• Katano, H., Pesnicak, L., Cohen, J. I. (2004). Simvastatin induces apoptosis of Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines and delays development of EBV lymphomas. Proc. Natl. Acad. Sci. USA 101: 4960-4965 [Abstract] [Full Text]  
• Kevil, C. G., Chidlow, J. H., Bullard, D. C., Kucik, D. F. (2003). High-temporal-resolution analysis demonstrates that ICAM-1 stabilizes WEHI 274.1 monocytic cell rolling on endothelium. Am. J. Physiol. Cell Physiol. 285: C112-C118 [Abstract] [Full Text]  
• Salas, A., Shimaoka, M., Chen, S., Carman, C. V., Springer, T. (2002). Transition From Rolling to Firm Adhesion Is Regulated by the Conformation of the I Domain of the Integrin Lymphocyte Function-associated Antigen-1. J. Biol. Chem. 277: 50255-50262 [Abstract] [Full Text]  
• DiVietro, J. A., Smith, M. J., Smith, B. R. E., Petruzzelli, L., Larson, R. S., Lawrence, M. B. (2001). Immobilized IL-8 Triggers Progressive Activation of Neutrophils Rolling In Vitro on P-Selectin and Intercellular Adhesion Molecule-1. J. Immunol. 167: 4017-4025 [Abstract] [Full Text]  
• Henderson, R. B., Lim, L. H.K., Tessier, P. A., Gavins, F. N.E., Mathies, M., Perretti, M., Hogg, N. (2001). The Use of Lymphocyte Function-Associated Antigen (Lfa)-1-Deficient Mice to Determine the Role of Lfa-1, Mac-1, and {alpha}4 Integrin in the Inflammatory Response of Neutrophils. JEM 194: 219-226 [Abstract] [Full Text]  
• Singbartl, K., Thatte, J., Smith, M. L., Wethmar, K., Day, K., Ley, K. (2001). A CD2-Green Fluorescence Protein-Transgenic Mouse Reveals Very Late Antigen-4-Dependent CD8+ Lymphocyte Rolling in Inflamed Venules. J. Immunol. 166: 7520-7526 [Abstract] [Full Text]  
• Lu, C., Shimaoka, M., Ferzly, M., Oxvig, C., Takagi, J., Springer, T. A. (2001). An isolated, surface-expressed I domain of the integrin alpha Lbeta 2 is sufficient for strong adhesive function when locked in the open conformation with a disulfide bond. Proc. Natl. Acad. Sci. USA 98: 2387-2392 [Abstract] [Full Text]  
• Lu, C., Shimaoka, M., Zang, Q., Takagi, J., Springer, T. A. (2001). Locking in alternate conformations of the integrin alpha Lbeta 2 I domain with disulfide bonds reveals functional relationships among integrin domains. Proc. Natl. Acad. Sci. USA 98: 2393-2398 [Abstract] [Full Text]  
• Orchekowski, R. P., Plescia, J., Altieri, D. C., Bajt, M. L. (2000). {alpha}M{beta}2 (CD11b/CD18, Mac-1) integrin activation by a unique monoclonal antibody to {alpha}M I domain that is divalent cation-sensitive. J. Leukoc. Biol. 68: 641-649 [Abstract] [Full Text]  
• Sigal, A., Bleijs, D. A., Grabovsky, V., van Vliet, S. J., Dwir, O., Figdor, C. G., van Kooyk, Y., Alon, R. (2000). The LFA-1 Integrin Supports Rolling Adhesions on ICAM-1 Under Physiological Shear Flow in a Permissive Cellular Environment. J. Immunol. 165: 442-452 [Abstract] [Full Text]  
• Kunkel, E. J., Dunne, J. L., Ley, K. (2000). Leukocyte Arrest During Cytokine-Dependent Inflammation In Vivo. J. Immunol. 164: 3301-3308 [Abstract] [Full Text]  
• Mizgerd, J. P., Bullard, D. C., Hicks, M. J., Beaudet, A. L., Doerschuk, C. M. (1999). Chronic Inflammatory Disease Alters Adhesion Molecule Requirements for Acute Neutrophil Emigration in Mouse Skin. J. Immunol. 162: 5444-5448 [Abstract] [Full Text]  
• Edwards, C. P., Fisher, K. L., Presta, L. G., Bodary, S. C. (1998). Mapping the Intercellular Adhesion Molecule-1 and -2 Binding Site on the Inserted Domain of Leukocyte Function-associated Antigen-1. J. Biol. Chem. 273: 28937-28944 [Abstract] [Full Text]  
• Dustin, M. L., Golan, D. E., Zhu, D.-M., Miller, J. M., Meier, W., Davies, E. A., van der Merwe, P. A. (1997). Low Affinity Interaction of Human or Rat T Cell Adhesion Molecule CD2 with Its Ligand Aligns Adhering Membranes to Achieve High Physiological Affinity. J. Biol. Chem. 272: 30889-30898 [Abstract] [Full Text]  
• Yalamanchili, P., Lu, C., Oxvig, C., Springer, T. A. (2000). Folding and Function of I Domain-deleted Mac-1 and Lymphocyte Function-associated Antigen-1. J. Biol. Chem. 275: 21877-21882 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS