The Journal of Experimental Medicine
VeriKine-HS Human IFN-Beta
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© The Rockefeller University Press, 0022-1007/1997/8/665/ $5.00
The Journal of Experimental Medicine, Volume 186, Number 5, August 29, 1997 665-672

Article

High Levels of a Major Histocompatibility Complex II–Self Peptide Complex on Dendritic Cells from the T Cell Areas of Lymph Nodes

Kayo Inaba{ddagger}, Maggie Pack*, Muneo Inaba§, Hiraki Sakuta{ddagger}, Frank Isdell*, and Ralph M. Steinman*

From * The Rockefeller University, New York 10021; {ddagger} Kyoto University, Kitashirakawa-Oiusake-cho, Kyoto 606-01, Japan; and § Kansai Medical University, Fumizono-cho, Osaka 576, Japan

T lymphocytes recirculate continually through the T cell areas of peripheral lymph nodes. During each passage, the T cells survey the surface of large dendritic cells (DCs), also known as interdigitating cells. However, these DCs have been difficult to release from the lymph node. By emphasizing the use of calcium-free media, as shown by Vremec et al. (Vremec, D., M. Zorbas, R. Scollay, D.J. Saunders, C.F. Ardavin, L. Wu, and K. Shortman. 1992. J. Exp. Med. 176:47–58.), we have been able to release and enrich DCs from the T cell areas. The DCs express the CD11c leukocyte integrin, the DEC-205 multilectin receptor for antigen presentation, the intracellular granule antigens which are recognized by monoclonal antibodies M342, 2A1, and MIDC-8, very high levels of MHC I and MHC II, and abundant accessory molecules such as CD40, CD54, and CD86. When examined with the Y-Ae monoclonal which recognizes complexes formed between I-Ab and a peptide derived from I-E{alpha}, the T cell area DCs expressed the highest levels. The enriched DCs also stimulated a T-T hybridoma specific for this MHC II–peptide complex, and the hybridoma underwent apoptosis. Therefore DCs within the T cell areas can be isolated. Because they present very high levels of self peptides, these DCs should be considered in the regulation of self reactivity in the periphery.

Address correspondence to Dr. Ralph M. Steinman, The Rockefeller University, 1230 York Avenue, New York, NY 10021. Phone: 212-327-8106; FAX: 212-327-8875; E-mail:steinma{at}rockvax.rockefeller.edu

Abbreviations used: DC, dendritic cell.


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