The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 621K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mueller, C. G.F.
Right arrow Articles by Liu, Y.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mueller, C. G.F.
Right arrow Articles by Liu, Y.-J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/1997/8/655/ $5.00
The Journal of Experimental Medicine, Volume 186, Number 5, August 29, 1997 655-663

Article

Polymerase Chain Reaction Selects a Novel Disintegrin Proteinase from CD40-Activated Germinal Center Dendritic Cells

Chris G.F. Mueller, Marie-Clotilde Rissoan, Barbara Salinas, Smina Ait-Yahia, Odile Ravel, Jean-Michel Bridon, Francine Briere, Serge Lebecque, and Yong-Jun Liu

From the Schering-Plough Laboratory for Immunological Research, 69571 Dardilly, France

To identify genes expressed by a specific subset of dendritic cells found in vivo a polymerase chain reaction–based cDNA subtraction technique was applied to the recently described germinal center dendritic cells. A novel member of the disintegrin metalloproteinase family was cloned which comprises a not typical zinc-chelating catalytic site most similar to a bacterial metalloproteinase. Dendritic cell precursors or immature dendritic cells express no or low levels of the message. It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction. In situ hybridization showed distinct expression of this gene in the germinal center. This, together with the findings that certain disintegrin metalloproteinases regulate the activity of tumor necrosis factor {alpha} and that metalloproteinases have also been implicated in FasL processing, suggest that this novel molecule may play an important role in dendritic cell function and their interactions with germinal center T cells.

Address correspondence to Dr. Yong-Jun Liu, Schering-Plough, Laboratory for Immunological Reseach, 27 chemin des Peupliers, BP11, 69571 Dardilly, France. Phone: 33-4-72-17-27-00; FAX: 33-4-78-35-47-50.

Note added in proof. DC tactin is identical to macrophage-derived chemokine recently published (Godiska et al., J. Exp. Med. 185:1595–1604.

1 Abbreviations used in this paper: DC, dendritic cell; GCDC, germinal center DC; mRNA, messenger RNA; RACE, rapid amplification of cohesive ends; RT-PCR, PCR coupled to reverse transcribed RNA.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS