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© The Rockefeller University Press, 0022-1007/1997/8/645/ $5.00
The Journal of Experimental Medicine, Volume 186, Number 5, August 29, 1997 645-653

Article

Self Antigens Expressed by Solid Tumors Do Not Efficiently Stimulate Naive or Activated T Cells: Implications for Immunotherapy

Daniel E. Speiser*, Renata Miranda*, Arsen Zakarian*, Martin F. Bachmann*, Kim McKall-Faienza*, Bernhard Odermatt§, Douglas Hanahan{ddagger}, Rolf M. Zinkernagel§, and Pamela S. Ohashi*

From the * Ontario Cancer Institute, Department of Medical Biophysics and Department of Immunology, University of Toronto, Ontario M5G 2M9, Canada; {ddagger} Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0534; and § Institute for Experimental Immunology, University Hospital of Zürich, CH-8091 Zürich, Switzerland

Induction and maintenance of cytotoxic T lymphocyte (CTL) activity specific for a primary endogenous tumor was investigated in vivo. The simian virus 40 T antigen (Tag) expressed under the control of the rat insulin promoter (RIP) induced pancreatic β-cell tumors producing insulin, causing progressive hypoglycemia. As an endogenous tumor antigen, the lymphocytic choriomeningitis virus (LCMV) glycoprotein (GP) was introduced also under the control of the RIP. No significant spontaneous CTL activation against GP was observed. However, LCMV infection induced an antitumor CTL response which efficiently reduced the tumor mass, resulting in temporarily normalized blood glucose levels and prolonged survival of double transgenic RIP(GP x Tag2) mice (137 ± 18 d) as opposed to control RIP-Tag2 mice (88 ± 8 d). Surprisingly, the tumor-specific CTL response was not sustained despite the facts that the tumor cells continued to express MHC class I and LCMV-GP–specific CTLs were present and not tolerized. Subsequent adoptive transfer of virus activated spleen cells into RIP(GP x Tag2) mice further prolonged survival (168 ± 11 d), demonstrating continued expression of the LCMV-GP tumor antigen and MHC class I. The data show that the tumor did not spontaneously induce or maintain an activated CTL response, revealing a profound lack of immunogenicity in vivo. Therefore, repetitive immunizations are necessary for prolonged antitumor immunotherapy. In addition, the data suggest that the risk for induction of chronic autoimmune diseases is limited, which may encourage immunotherapy against antigens selectively but not exclusively expressed by the tumor.

Address correspondence to Daniel E. Speiser, Ontario Cancer Institute, Departments of Medical Biophysics and Immunology, University of Toronto, 610 University Avenue, Toronto, Ontario M5G 2M9, Canada. Phone: 416-946-4501, extension 5470. FAX: 416-946-2086.

1 Abbreviations used in this paper: GP, glycoprotein; LCMV, lymphocytic choriomeningitis virus; RIP, rat insulin promoter; Tag, T antigen.


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