J. Exp. Med.
© The Rockefeller University Press
0022-1007/97/08/467/06 $2.00
Volume 186, Number 3, August 4, 1997 467-472

BRIEF DEFINITIVE REPORT:
Rat Spleen Dendritic Cells Express Natural Killer Cell Receptor Protein 1 (NKR-P1) and Have Cytotoxic Activity to Select Targets via a Ca²⁺-dependent Mechanism

By Régis Josien, Michèle Heslan, Jean-Paul Soulillou, and Maria-Cristina Cuturi

From the Institut National de la Sante et de la Recherche Medicale U437 and Institut de Transplantation et de Recherche en Transplantation, Immeuble Jean Monnet, 44093 Nantes Cédex 1, France

Dendritic cells (DC) are a subset of leukocytes whose major function is antigen presentation. We investigated the phenotype and function of enriched (95-98.5%) rat DC. We show that both spleen and thymus DC express the natural killer cell receptor protein 1 (NKR-P1) as a disulfide linked homodimer of 60 kD. Freshly isolated DC express a low level of NKR-P1, which is strongly upregulated after overnight culture. Spleen, but not thymus DC, were able to kill the NK-sensitive YAC-1 cell line in vitro, and since this killing was Ca²⁺ dependent, a Fas ligand-Fas interaction was probably not involved. Besides their potent antigen-presenting function, DC can thus be cytotoxic for some tumor targets.

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