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Article

Ulrich Martin^*, Daniel Bock^*, Lubomir Arseniev, Mark A. Tornetta, Robert S. Ames, Wilfried Bautsch^*, Jörg Köhl^*, Arnold Ganser, and Andreas Klos^*

From the ^* Institute of Medical Microbiology, Hannover Medical School; the Department of Hematology and Oncology, Hannover, 30625, Germany; and the Department of Molecular Immunology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939

The pathophysiological relevance of the complement split product C3a as a proinflammatory mediator is still ill defined. The expression pattern of the human C3a receptor (C3aR) can provide important clues for the role of this anaphylatoxin in inflammation. There is strong evidence for C3aR expression on basophils, and eosinophils, but additionally, only on tumor cell lines of leukemic or hepatic origin. It is unclear whether neutrophils also express the C3aR, but need a costimulus provided by eosinophils for certain biological responses, or whether neutrophils lack the C3aR and respond to C3a via a secondary stimulus generated by eosinophils, i.e., by an indirect mode. In the present study, polyclonal antiserum raised against the second extracellular loop of the C3aR was used to characterize C3aR expression on peripheral blood leukocytes. For high degree purification of neutrophils, a negative selection method was established that decreased the contamination with CD9^bright+ eosinophils down to <0.2%. Flow cytometric analyses, functional assays, and binding assays on highly purified neutrophils confirmed C3aR expression and coupling. Monocytes were identified as an additional C3aR-positive cell population of the peripheral blood. The expression of the C3aR on eosinophils could be confirmed. In contrast, the receptor could not be detected on unchallenged B or T lymphocytes (or lymphocyte-derived Raji cells).

¹ Abbreviations used in this paper: C3a, an anaphylatoxic peptide generated from the complement component C3; C3aR, C3a receptor; [Ca²⁺]_i, concentration of free cytosolic Ca²⁺, GAPDH, rat glyceraldehyde-3-phosphate dehydrogenase used as housekeeping gene.

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