The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1997/11/1713/ $5.00
The Journal of Experimental Medicine, Volume 186, Number 10, November 17, 1997 1713-1724

Articles

Regulation of ZAP-70 Intracellular Localization: Visualization with the Green Fluorescent Protein

Joanne Sloan-Lancaster*, Weiguo Zhang*, John Presley{ddagger}, Brandi L. Williams§, Robert T. Abraham§, Jennifer Lippincott-Schwartz{ddagger}, and Lawrence E. Samelson*

From * the Section on Lymphocyte Signaling and {ddagger} the Unit of Organelle Biology, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892; and § The Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905

To investigate the cellular dynamics of ZAP-70, we have studied the distribution and regulation of its intracellular location using a ZAP-70 green fluorescent protein chimera. Initial experiments in epithelial cells indicated that ZAP-70 is diffusely located throughout the quiescent cell, and accumulates at the plasma membrane upon cellular activation, a phenotype enhanced by the coexpression of Lck and the initiation of ZAP-70 kinase activity. Subsequent studies in T cells confirmed this phenotype. Intriguingly, a large amount of ZAP-70, both chimeric and endogenous, resides in the nucleus of quiescent and activated cells. Nuclear ZAP-70 becomes tyrosine phosphorylated upon stimulation via the T cell receptor, indicating that it may have an important biologic function.

Address correspondence to Lawrence E. Samelson, NICHD, CBMB, Bldg. 18T, Rm 101, Bethesda, MD 20892. Phone: 301-496-6368; FAX: 301-402-0078; E-mail: samelson{at}helix.nih.gov

Dr. Sloan-Lancaster is a fellow of the Damon-Runyon/Walter Winchell Cancer Research Fund. Dr. Zhang is supported by the Leukemia Society of America. Dr. Abraham is supported by a National Institutes of Health grant GM47286.

1 Abbreviations used in this paper: cfb3, cytosolic fragment of erythrocyte band 3; GFP, green fluorescent protein; IF, immunofluorescence; IP, immunoprecipitation; IRP, iron regulatory protein; ITAM, immunoreceptor tyrosine-based activation motif; KD, kinase dead; PTK, protein tyrosine kinase; PV, pervanadate; NLS, nuclear localization signal; ROI, region of interest; RT, room temperature.


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