J. Exp. Med.
© The Rockefeller University Press
0022-1007/97/07/91/10 $2.00
Volume 186, Number 1, July 7, 1997 91-100

Identification of a DNA Segment Exhibiting Rearrangement Modifying Effects upon Transgenic -deleting Elements

By Karen M. Janowski,^* Stephanie Ledbetter,^* Matthew S. Mayo, and Richard D. Hockett Jr.^*

From the ^* Department of Pathology, and the  Biostatistics Unit, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, Alabama 35233-7331

Control of the rearrangement and expression of the T cell receptor  and  chains is critical for determining T cell type. The process of  deletion is a candidate mechanism for maintaining separation of the  and  loci. Mice harboring a transgenic reporter  deletion construct show / T cell lineage-specific use of the transgenic elements. A 48-basepair segment of DNA, termed HPS1A, when deleted from this reporter construct, loses tight lineage-specific rearrangement control of transgenic elements, with abundant rearrangements of transgenic -deleting elements now in / T cells. Furthermore, HPS1A augments recombination frequency of extrachromosomal substrates in an in vitro recombination assay. DNA binding proteins recognizing HPS1A have been identified and are restricted to early B and T cells, during the time of active rearrangement of endogenous TCR and immunoglobulin loci. These data are consistent with  deletion playing an important role in maintaining separate TCR  and loci.

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