The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1997/2/777/ $5.00
The Journal of Experimental Medicine, Volume 185, Number 4, February 17, 1997 777-784

Articles

A Novel Migration Pathway for Rat Dendritic Cells from the Blood: Hepatic Sinusoids–Lymph Translocation

Shunsuke Kudo*,{ddagger}, Kenjiro Matsuno*, Taichi Ezaki*, and Michio Ogawa{ddagger}

From the * Department of Anatomy II, and the {ddagger} Department of Surgery II, Kumamoto University School of Medicine, Kumamoto 860, Japan

The migration pathways for dendritic cells (DC) from the blood are not yet completely resolved. In our previous study, a selective recruitment of DC progenitors from the blood to the liver was suggested. To clarify the role of the hepatic sinusoids in the migration of blood DC, relatively immature DC and mature DC were isolated from hepatic and intestinal lymph, and intravenously transferred to allogeneic hosts. It was then possible to detect small numbers of DC within secondary lymphoid tissues either by immunostaining for donor type major histocompatibility complex class I antigen or, at much higher sensitivity, for bromodeoxyuridine incorporated by proliferating cells (mainly T lymphocytes), which responded to the alloantigen presented by the administered DC. The intravenously injected DC accumulated in the paracortex of regional lymph nodes of the liver via a lymph-borne pathway. Intravenously injected fluorochrome-labeled syngeneic DC behaved similarly. In contrast, very few DC were found in spleen sections and were hardly detectable in other lymph nodes or in other tissues. An in situ cell binding assay revealed a significant and selective binding of DC to Kupffer cells in liver cryosections. It is concluded that rat DC can undergo a blood–lymph translocation via the hepatic sinusoids, but not via the high endothelial venules of lymph nodes. Hence the hepatic sinusoids may act as a biological concentrator of blood DC into the regional hepatic nodes. Kupffer cells may play an important role in this mechanism.

Address correspondence to Dr. Kenjiro Matsuno, Department of Anatomy II, Kumamoto University School of Medicine, 2-2-1, Honjo, Kumamoto 860, Japan.

1Abbreviations used in this paper: BrdU, 5-bromo-2'-deoxyuridine; DC, dendritic cells.

Part of this work was presented at the 4th International Symposium on Dendritic Cells, Venice, Italy, October, 1996.


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