The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1997/2/653/ $5.00
The Journal of Experimental Medicine, Volume 185, Number 4, February 17, 1997 653-662

Articles

A Transgenic Marker for Mouse B Lymphoid Precursors

Inga-Lill Mårtensson*,{ddagger}, Fritz Melchers{ddagger}, and Thomas H. Winkler{ddagger}

From the * Department of Cellular and Molecular Biology, Immunology Group, S-223 62 Lund, Sweden; and the {ddagger} Basel Institute for Immunology, CH-4005 Basel, Switzerland

Three lines of transgenic mice have been generated which express human CD25 under the control of the 722-base pair region located immediately 5' of the precursor (pre)–B cell–specific {lambda}5 gene. All three strains express human CD25 in parallel to endogenous {lambda}5 on pre–B cells, but not on mature B lymphocytes or other blood cell lineages. High expression of human CD25 on B lineage cells of transgenic mice has allowed the identification of a new B220+CD19–{lambda}5+ precursor of the B220+CD19+{lambda}5+ c-kit+ pre-BI cells. Both types of precursors are clonable on stromal cells in the presence of interleukin-7. The CD19– precursors have a sizeable part of their immunoglobulin heavy chain gene loci in germline configuration, while the CD19+ pre–BI cells are predominantly DJH rearranged. The results indicate that random integration of the 722-bp 5' region of the {lambda}5 gene into the mouse genome confers tissue and differentiation stage–specific expression of a transgene.

Address correspondence to Fritz Melchers, Basel Institute for Immunology, Grenzacherstr. 487, CH-4005 Basel, Switzerland. T.H. Winkler's present address is Section of Immunology, Medical Department III, University of Erlangen-Nurnberg, Schwabachanlage 10, D-91054 Erlangen, Germany.

1 Abbreviations used in this paper: BM, bone marrow; HPRT, hypoxanthine phosphoribosyl transferase; HSA, heat stable antigen; hu-CD25, human CD25; pre, precursor; RT, reverse transcription.


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