The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1997/1/207/ $5.00
The Journal of Experimental Medicine, Volume 185, Number 2, January 20, 1997 207-218

Articles

Absence of Respiratory Burst in X-linked Chronic Granulomatous Disease Mice Leads to Abnormalities in Both Host Defense and Inflammatory Response to Aspergillus fumigatus

David E. Morgenstern*, Mary A.C. Gifford*, Ling Lin Li*, Claire M. Doerschuk{ddagger}, and Mary C. Dinauer*

From the * Herman B Wells Center for Pediatric Research, Department of Pediatrics (Hematology-Oncology), James Whitcomb Riley Hospital for Children, Indiana University Medical Center, Indianapolis, Indiana 46202; and {ddagger} Physiology Program, Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115

Mice with X-linked chronic granulomatous disease (CGD) generated by targeted disruption of the gp91phox subunit of the NADPH–oxidase complex (X-CGD mice) were examined for their response to respiratory challenge with Aspergillus fumigatus. This opportunistic fungal pathogen causes infection in CGD patients due to the deficient generation of neutrophil respiratory burst oxidants important for damaging A. fumigatus hyphae. Alveolar macrophages from X-CGD mice were found to kill A. fumigatus conidia in vitro as effectively as alveolar macrophages from wild-type mice. Pulmonary disease in X-CGD mice was observed after administration of doses ranging from 105 to 48 spores, none of which produced disease in wild-type mice. Higher doses produced a rapidly fatal bronchopneumonia in X-CGD mice, whereas progression of disease was slower at lower doses, with development of chronic inflammatory lesions. Marked differences were also observed in the response of X-CGD mice to the administration of sterilized Aspergillus hyphae into the lung. Within 24 hours of administration, X-CGD mice had significantly higher numbers of alveolar neutrophils and increased expression of the proinflammatory cytokines IL-1β and TNF-{alpha} relative to the responses seen in wild-type mice. By one week after administration, pulmonary inflammation was resolving in wild-type mice, whereas X-CGD mice developed chronic granulomatous lesions that persisted for at least six weeks. This is the first experimental evidence that chronic inflammation in CGD does not always result from persistent infection, and suggests that the clinical manifestations of this disorder reflect both impaired microbial killing as well as other abnormalities in the inflammatory response in the absence of a respiratory burst.

Address correspondence to Dr. Mary C. Dinauer, Herman B. Wells Center for Pediatric Research, James Whitcomb Riley Hospital for Children, 702 Barnhill Dr., Indianapolis, IN 46202.

The authors would like to thank Attilio Orazi and Karla John for preparation of histology sections and Jim Cumming for assistance in mouse colony maintenance.

This work was supported by National Institutes of Health grant R01 HL52565 and a Biomedical Research grant from the Arthritis Foundation.

1Abbreviations used in this paper: cfu, colony-forming units; CGD, chronic granulomatous disease.


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