The Journal of Experimental Medicine
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 134K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tough, D. F.
Right arrow Articles by Sprent, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tough, D. F.
Right arrow Articles by Sprent, J.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/1997/6/2089/ $5.00
The Journal of Experimental Medicine, Volume 185, Number 12, June 16, 1997 2089-2094

Articles

T Cell Stimulation In Vivo by Lipopolysaccharide (LPS)

David F. Tough, Siquan Sun, and Jonathan Sprent

From the Department of Immunology, The Scripps Research Institute, La Jolla, California 92037

Lipopolysaccharide (LPS) from gram-negative bacteria causes polyclonal activation of B cells and stimulation of macrophages and other APC. We show here that, under in vivo conditions, LPS also induces strong stimulation of T cells. As manifested by CD69 upregulation, LPS injection stimulates both CD4 and CD8+ T cells, and, at high doses, stimulates naive (CD44lo) cells as well as memory (CD44hi) cells. However, in terms of cell division, the response of T cells after LPS injection is limited to the CD44hi subset of CD8+ cells. In contrast with B cells, proliferative responses of CD44hi CD8+ cells require only very low doses of LPS (10 ng). Based on studies with LPS-nonresponder and gene-knockout mice, LPS-induced proliferation of CD44hi CD8+ cells appears to operate via an indirect pathway involving LPS stimulation of APC and release of type I ({alpha}, β) interferon (IFN-I). Similar selective stimulation of CD44hi CD8+ cells occurs in viral infections and after injection of IFN-I, implying a common mechanism. Hence, intermittent exposure to pathogens (gram-negative bacteria and viruses) could contribute to the high background proliferation of memory–phenotype CD8+ cells found in normal animals.

Address correspondence to Jonathan Sprent, Department of Immunology, IMM4, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037.

1Abbreviations used in this paper: BrdU, bromodeoxyuridine; IFN-I, type I ({alpha}, β) interferon; LCMV, lymphocytic choriomeningitis virus.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS