The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article
Right arrow Full Text
Right arrow Full Text (PDF, 237K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aiuti, A.
Right arrow Articles by Gutierrez-Ramos, J.C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aiuti, A.
Right arrow Articles by Gutierrez-Ramos, J.C.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/1997/1/111/ $5.00
The Journal of Experimental Medicine, Volume 185, Number 1, January 6, 1997 111-120


Articles

The Chemokine SDF-1 Is a Chemoattractant for Human CD34+ Hematopoietic Progenitor Cells and Provides a New Mechanism to Explain the Mobilization of CD34+ Progenitors to Peripheral Blood

A. Aiuti*,{ddagger}, I.J. Webb||, C. Bleul{ddagger}, T. Springer{ddagger}, and J.C. Gutierrez-Ramos*,{ddagger},**

From the * Center for Blood Research, Inc., and the {ddagger} Department of Genetics, § Department of Pathology, and || Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; Blood Component Laboratory, Dana-Farber Cancer Institute, Boston, Massachusetts 02115; and ** Millennium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139

Hematopoietic progenitor cells migrate in vitro and in vivo towards a gradient of the chemotactic factor stromal cell-derived factor-1 (SDF-1) produced by stromal cells. This is the first chemoattractant reported for human CD34+ progenitor cells. Concentrations of SDF-1 that elicit chemotaxis also induce a transient elevation of cytoplasmic calcium in CD34+ cells. SDF-1-induced chemotaxis is inhibited by pertussis toxin, suggesting that its signaling in CD34+ cells is mediated by seven transmembrane receptors coupled to Gi proteins. CD34+ cells migrating to SDF-1 include cells with a more primitive (CD34+/CD38 or CD34+/DR) phenotype as well as CD34+ cells phenotypically committed to the erythroid, lymphoid and myeloid lineages, including functional BFU-E, CFU-GM, and CFU-MIX progenitors. Chemotaxis of CD34+ cells in response to SDF-1 is increased by IL-3 in vitro and is lower in CD34+ progenitors from peripheral blood than in CD34+ progenitors from bone marrow, suggesting that an altered response to SDF-1 may be associated with CD34 progenitor mobilization.


Address correspondence to J.C. Gutierrez-Ramos, Millenium Pharmaceuticals, Inc., 640 Memorial Drive, Cambridge, MA 02139-4815.

The authors are indebted to Dr. Van Etten for critical reading of this manuscript and to Drs. Arman, Alvarez de Mon, and Alvarez for their efforts in locating invaluable PBPC and BM samples. We thank especially Dr. Anderson for helpful discussions and Drs. Mazo and Gerwin for their help.

This work has been funded by National Institutes of Health grants HL 148675-02 and HL94-10-B, and by the Aplastic Foundation of America grants CiCyT PB93-0317. J.C. Gutierrez-Ramos is the Amy C. Potter fellow. A. Aiuti's present address is TIGET, Instituto Scientifico San Raffaele, Via Olgettima 58, 20132, Milano, Italy.

1Abbreviations used in this paper: BM, bone marrow; CFC, colony-forming cell; FL, fetal liver; G-CSF, granulocyte–colony-stimulating factor; HPC, hematopoietic progenitor cells; HS, Hepes-buffered saline; MCP, macrophage chemotactic protein; MIP, macrophage inflammatory protein; PBPC, peripheral blood progenitor cell; PI, propidium iodide; SDF, stromal cell-derived factor.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS