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Genes Is
Blocked in Interleukin-7 Receptor-deficient Mice
By
From the * Department of Disease-related Gene Regulation Research (Sandoz), Faculty of Medicine,
The University of Tokyo, Tokyo 113, Japan; and the IL-7R-deficient mice have severely impaired expansion of early lymphocytes and lack Department of Medical Chemistry, Faculty of
Medicine, Kyoto University, Kyoto 606, Japan
T cells.
To elucidate the role of IL-7R on T cell development, we analyzed the rearrangements of
TCR-
, 
, , and genes in the thymus of the IL-7R-deficient mice. Southern blot analysis
with a J1 probe revealed that more than 70% of J1 and J2 alleles are recombined to form
distinct V1.2-J2 and V2-J1 fragments in control mice. On the contrary, no such recombination was detected in the mutant mice. The rearrangements in the TCR-, , and loci
were comparably observed in control and mutant mice. PCR analysis indicated that the V-J
recombination of all the V genes is severely hampered in the mutant mice. The mRNA of
RAG-1, RAG-2, Ku-80, and terminal deoxynucleotidyl transferase (TdT) genes was equally
detected between control and mutant thymi, suggesting that the expression of common recombination machinery is not affected. These data demonstrated that the V-J recombination of
the TCR genes is specifically blocked in the IL-7R-deficient mice and suggested the presence of highly specific regulation for TCR gene rearrangement.
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