© The Rockefeller University Press, 0022-1007/1996/12/2207/ $5.00
The Journal of Experimental Medicine, Volume 184, Number 6, December 1, 1996 2207-2216
Identification of TRP-2 as a Human Tumor Antigen Recognized by Cytotoxic T Lymphocytes
Rong-Fu Wang,
Ettore Appella,
Yutaka Kawakami,
Xiaoqiang Kang, and
Steven A. Rosenberg
From the National Cancer Institute, Bethesda, Maryland 20892
The infusion of TIL586 along with interleukin-2 into the autologous patient with metastatic melanoma resulted in the objective regression of tumor. A gene encoding a tumor antigen recognized by TIL586 was previously isolated and shown to encode gp75 or TRP-1. Here we report that TRP-2 was identified as a second tumor antigen recognized by a HLA-A31– restricted CTL clone derived from the TIL586 cell line. The peptide LLPGGRPYR epitope was subsequently identified from the coding region of TRP-2 based on studies of the recognition of truncated TRP-2 cDNAs and the HLA-A31 binding motif. This epitope peptide was capable of sensitizing target cells for lysis by a CTL clone at 1 nM peptide concentration. Although some modified peptides could be recognized by the CTL clone, none were found to be better recognized by T cells than the parental peptide. Like other melamona differentiation antigens, TRP-2 was only expressed in melanoma, melanocytes, and retina, but not in other human tissues tested.
Address correspondence to Dr. Rong-Fu Wang, Building 10, 2B42, National Cancer Institute, NIH, Bethesda, MD 20892-1502.
1Abbreviations used in this paper: E/T, effector/target ratio; TIL, tumor infiltrating lymphocyte.

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