Inflammatory microcrystals differentially regulate the secretion of macrophage inflammatory protein 1 and interleukin 8 by human neutrophils: a possible mechanism of neutrophil recruitment to sites of inflammation in synovitis

doi:10.1084/jem.182.6.2019

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Inflammatory microcrystals differentially regulate the secretion of macrophage inflammatory protein 1 and interleukin 8 by human neutrophils: a possible mechanism of neutrophil recruitment to sites of inflammation in synovitis

M Hachicha, PH Naccache and SR McColl
Department of Microbiology and Immunology, University of Adelaide, South Australia.

Human neutrophils at inflammatory sites may be an important source of the chemotactic cytokines macrophage inflammatory protein 1 alpha (M1P- 1 alpha; a C-C chemokine) and interleukin 8 (IL-8; a C-X-C chemokine). In this study, we show that the inflammatory microcrystals monosodium urate monohydrate (MSU) and calcium pyrophosphate dihydrate (CPPD), the major mediators of gout and pseudogout, differentially regulate the production of these two chemokines by human neutrophils. Both MSU and CPPD increased the secretion of IL-8 by neutrophils in a dose- and time- dependent manner, but had no effect on that of MIP-1 alpha. Since inflammatory cytokines are likely to be present in the synovium during crystal-induced inflammation, we examined the interaction between TNF- alpha and GM-CSF and the crystals. Both TNF-alpha and GM-CSF stimulated IL-8 production; however, only TNF-alpha exerted a significant effect on MIP-1 alpha secretion in neutrophils. IL-8 production induced by TNF- alpha and GM-CSF was synergistically enhanced in the presence of MSU or CPPD, whereas MIP-1 alpha secretion induced by TNF was completely inhibited in the presence of either MSU or CPPD. Interestingly, no interaction between the crystals and the inflammatory cytokines was observed with respect to synthesis of the C-X-C chemokine MGSA in neutrophils. These results suggest that the combination of TNF-alpha and GM-CSF with MSU or CPPD will lead to the production of IL-8 by neutrophils and abolish the release of MIP-1 alpha, an event that will theoretically lead to recruitment of neutrophils but not mononuclear cells. These results are in accordance with the pathological state of gout and pseudogout, where the predominant inflammatory cell is the neutrophil.
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Getting, S. J., Gibbs, L., Clark, A. J. L., Flower, R. J, Perretti, M. (1999). POMC Gene-Derived Peptides Activate Melanocortin Type 3 Receptor on Murine Macrophages, Suppress Cytokine Release, and Inhibit Neutrophil Migration in Acute Experimental Inflammation. J. Immunol. 162: 7446-7453 [Abstract] [Full Text]
Siddiqui, R. A., Akard, L. P., Garcia, J. G. N., Cui, Y., English, D. (1999). Chemotactic Migration Triggers IL-8 Generation in Neutrophilic Leukocytes. J. Immunol. 162: 1077-1083 [Abstract] [Full Text]
McColl, S. R., Staykova, M. A., Wozniak, A., Fordham, S., Bruce, J., Willenborg, D. O. (1998). Treatment with Anti-Granulocyte Antibodies Inhibits the Effector Phase of Experimental Autoimmune Encephalomyelitis. J. Immunol. 161: 6421-6426 [Abstract] [Full Text]
Tessier, P. A., Naccache, P. H., Diener, K. R., Gladue, R. P., Neote, K. S., Clark-Lewis, I., McColl, S. R. (1998). Induction of Acute Inflammation In Vivo by Staphylococcal Superantigens. II. Critical Role for Chemokines, ICAM-1, and TNF-{alpha}. J. Immunol. 161: 1204-1211 [Abstract] [Full Text]
Roberge, C. J., McColl, S. R., Larochelle, B., Gosselin, J. (1998). Granulocyte-Macrophage Colony-Stimulating Factor Enhances EBV-Induced Synthesis of Chemotactic Factors in Human Neutrophils. J. Immunol. 160: 2442-2448 [Abstract] [Full Text]
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Hachicha, M., Rathanaswami, P., Naccache, P. H., McColl, S. R. (1998). Regulation of Chemokine Gene Expression in Human Peripheral Blood Neutrophils Phagocytosing Microbial Pathogens. J. Immunol. 160: 449-454 [Abstract] [Full Text]
Getting, S. J., Flower, R. J., Parente, L., Médicis, R. d., Lussier, A., Woliztky, B. A., Martins, M. A., Perretti, M. (1997). Molecular Determinants of Monosodium Urate Crystal-Induced Murine Peritonitis: A Role for Endogenous Mast Cells and a Distinct Requirement for Endothelial-Derived Selectins. J. Pharmacol. Exp. Ther. 283: 123-130 [Abstract] [Full Text]