Cross-linking of major histocompatibility complex class II molecules by staphylococcal enterotoxin A superantigen is a requirement for inflammatory cytokine gene expression

doi:10.1084/jem.182.5.1573

This Article

	Full Text (PDF, 652K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Mehindate, K.
	Articles by Mourad, W.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Mehindate, K.
	Articles by Mourad, W.


Social Bookmarking

	What's this?

ARTICLES

Cross-linking of major histocompatibility complex class II molecules by staphylococcal enterotoxin A superantigen is a requirement for inflammatory cytokine gene expression

K Mehindate, J Thibodeau, M Dohlsten, T Kalland, RP Sekaly and W Mourad
Centre de Recherche en Rhumatologie Immunologie, Le Centre Hospitalier de l'Universite Laval, Sainte-Foy, Quebec, Canada.

Staphylococcal enterotoxin A (SEA) has two distinct binding sites for major histocompatibility complex (MHC) class II molecules. The aspartic acid located at position 227 (D227) in the COOH terminus of SEA is one of the three residues involved in its interaction with the DR beta chain, whereas the phenylalanine 47 (F47) of the NH2 terminus is critical for its binding to the DR alpha chain. Upon interaction with MHC class II molecules, SEA triggers several cellular events leading to cytokine gene expression. In the present study, we have demonstrated that, contrary to wild-type SEA, stimulation of the THP1 monocytic cell line with SEA mutated at position 47 (SEAF47A) or at position 227 (SEAD227A) failed to induce interleukin 1 beta and tumor necrosis factor-alpha messenger RNA expression. Pretreatment of the cells with a 10-fold excess of either SEAF47A or SEAD227A prevented the increase in cytokine messenger RNA induced by wild-type SEA. However, cross-linking of SEAF47A or SEAD227A bound to MHC class II molecules with F(ab')2 anti-SEA mAb leads to cytokine gene expression, whereas cross-linking with F(ab) fragments had no effect. Taken together, these results indicate that cross-linking of two MHC class II molecules by one single SEA molecule is a requirement for cytokine gene expression.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Hopkins, P. A., Fraser, J. D., Pridmore, A. C., Russell, H. H., Read, R. C., Sriskandan, S. (2005). Superantigen recognition by HLA class II on monocytes up-regulates toll-like receptor 4 and enhances proinflammatory responses to endotoxin. Blood 105: 3655-3662 [Abstract] [Full Text]
Becart, S., Setterblad, N., Ostrand-Rosenberg, S., Ono, S. J., Charron, D., Mooney, N. (2003). Intracytoplasmic domains of MHC class II molecules are essential for lipid-raft-dependent signaling. J. Cell Sci. 116: 2565-2575 [Abstract] [Full Text]
Langlois, M.-A., Fakhry, Y. E., Mourad, W. (2003). Zinc-binding Sites in the N Terminus of Mycoplasma arthritidis-derived Mitogen Permit the Dimer Formation Required for High Affinity Binding to HLA-DR and for T Cell Activation. J. Biol. Chem. 278: 22309-22315 [Abstract] [Full Text]
Petersson, K., Pettersson, H., Skartved, N. J., Walse, B., Forsberg, G. (2003). Staphylococcal Enterotoxin H Induces V{alpha}-Specific Expansion of T Cells. J. Immunol. 170: 4148-4154 [Abstract] [Full Text]
Bouillon, M., El Fakhry, Y., Girouard, J., Khalil, H., Thibodeau, J., Mourad, W. (2003). Lipid Raft-dependent and -independent Signaling through HLA-DR Molecules. J. Biol. Chem. 278: 7099-7107 [Abstract] [Full Text]
Lei, L., Altstaedt, J., von der Ohe, M., Proft, T., Gross, U., Rink, L. (2001). Induction of interleukin-8 in human neutrophils after MHC class II cross-linking with superantigens. J. Leukoc. Biol. 70: 80-86 [Abstract] [Full Text]
Proft, T., Arcus, V. L., Handley, V., Baker, E. N., Fraser, J. D. (2001). Immunological and Biochemical Characterization of Streptococcal Pyrogenic Exotoxins I and J (SPE-I and SPE-J) from Streptococcus pyogenes. J. Immunol. 166: 6711-6719 [Abstract] [Full Text]
Matsuoka, T., Tabata, H., Matsushita, S. (2001). Monocytes Are Differentially Activated Through HLA-DR, -DQ, and -DP Molecules Via Mitogen-Activated Protein Kinases. J. Immunol. 166: 2202-2208 [Abstract] [Full Text]
Bobbitt, K. R., Justement, L. B. (2000). Regulation of MHC Class II Signal Transduction by the B Cell Coreceptors CD19 and CD22. J. Immunol. 165: 5588-5596 [Abstract] [Full Text]
Falk, K., Rotzschke, O., Santambrogio, L., Dorf, M. E., Brosnan, C., Strominger, J. L. (2000). Induction and Suppression of an Autoimmune Disease by Oligomerized T Cell Epitopes: Enhanced in Vivo Potency of Encephalitogenic Peptides. JEM 191: 717-730 [Abstract] [Full Text]
Cavallin, A., Arozenius, H., Kristensson, K., Antonsson, P., Otzen, D. E., Bjork, P., Forsberg, G. (2000). The Spectral and Thermodynamic Properties of Staphylococcal Enterotoxin A, E, and Variants Suggest That Structural Modifications Are Important to Control Their Function. J. Biol. Chem. 275: 1665-1672 [Abstract] [Full Text]
Aubert, V., Schneeberger, D., Sauty, A., Winter, J., Sperisen, P., Aubert, J.-D., Spertini, F. (2000). Induction of Tumor Necrosis Factor Alpha and Interleukin-8 Gene Expression in Bronchial Epithelial Cells by Toxic Shock Syndrome Toxin 1. Infect. Immun. 68: 120-124 [Abstract] [Full Text]
Nilsson, H., Bjork, P., Dohlsten, M., Antonsson, P. (1999). Staphylococcal Enterotoxin H Displays Unique MHC Class II-Binding Properties. J. Immunol. 163: 6686-6693 [Abstract] [Full Text]
Wright, A. D., Chapes, S. K. (1999). LPS sensitivity in recombinant mice lacking functional alleles at MHCII, Lps and Nramp1 genes. Innate Immunity 5: 297-305 [Abstract]
Huby, R. D. J., Dearman, R. J., Kimber, I. (1999). Intracellular Phosphotyrosine Induction by Major Histocompatibility Complex Class II Requires Co-aggregation with Membrane Rafts. J. Biol. Chem. 274: 22591-22596 [Abstract] [Full Text]
Leveille, C., Zekki, H., Al-Daccak, R., Mourad, W. (1999). CD40- and HLA-DR-mediated cell death pathways share a lot of similarities but differ in their use of ADP-ribosyltransferase activities. Int Immunol 11: 719-730 [Abstract] [Full Text]
Proft, T., Fraser, J. (1998). Superantigens: Just Like Peptides Only Different. JEM 187: 819-821 [Full Text]
Al-Daccak, R., Mehindate, K., Damdoumi, F., Etongue-Mayer, P., Nilsson, H., Antonsson, P., Sundstrom, M., Dohlsten, M., Sekaly, R.-P., Mourad, W. (1998). Staphylococcal Enterotoxin D Is a Promiscuous Superantigen Offering Multiple Modes of Interactions With the MHC Class II Receptors. J. Immunol. 160: 225-232 [Abstract] [Full Text]
Rotzschke, O., Falk, K., Strominger, J. L. (1997). Superactivation of an immune response triggered by oligomerized T cell�epitopes. Proc. Natl. Acad. Sci. USA 94: 14642-14647 [Abstract] [Full Text]
Li, P.-L., Tiedemann, R. E., Moffat, S. L., Fraser, J. D. (1997). The Superantigen Streptococcal Pyrogenic Exotoxin C (SPE-C) Exhibits a Novel Mode of Action. JEM 186: 375-383 [Abstract] [Full Text]
Hansson, J., Ohlsson, L., Persson, R., Andersson, G., Ilback, N.-G., Litton, M. J., Kalland, T., Dohlsten, M. (1997). Genetically engineered superantigens as tolerable antitumor�agents. Proc. Natl. Acad. Sci. USA 94: 2489-2494 [Abstract] [Full Text]
Tabata, H., Matsuoka, T., Endo, F., Nishimura, Y., Matsushita, S. (2000). Ligation of HLA-DR Molecules on B Cells Induces Enhanced Expression of IgM Heavy Chain Genes in Association with Syk Activation. J. Biol. Chem. 275: 34998-35005 [Abstract] [Full Text]